Altunbek Mine, Kuku Gamze, Culha Mustafa
Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Atasehir, Istanbul 34755, Turkey.
Molecules. 2016 Nov 25;21(12):1617. doi: 10.3390/molecules21121617.
The need for new therapeutic approaches in the treatment of challenging diseases such as cancer, which often consists of a highly heterogeneous and complex population of cells, brought up the idea of analyzing single cells. The development of novel techniques to analyze single cells has been intensively studied to fully understand specific alternations inducing abnormalities in cellular function. One of the techniques used for single cell analysis is surface-enhanced Raman spectroscopy (SERS) in which a noble metal nanoparticle is used to enhance Raman scattering. Due to its low toxicity and biocompatibility, gold nanoparticles (AuNPs) are commonly preferred as SERS substrates in single cell analysis. The intracellular uptake, localization and toxicity issues of AuNPs are the critical points for interpretation of data since the obtained SERS signals originate from molecules in close vicinity to AuNPs that are taken up by the cells. In this review, the AuNP-living cell interactions, cellular uptake and toxicity of AuNPs in relation to their physicochemical properties, and surface-enhanced Raman scattering from single cells are discussed.
对于诸如癌症等具有挑战性疾病的治疗，需要新的治疗方法，这些疾病通常由高度异质和复杂的细胞群体组成，这催生了分析单细胞的想法。为了充分理解导致细胞功能异常的特定变化，人们对分析单细胞的新技术开展了深入研究。用于单细胞分析的技术之一是表面增强拉曼光谱（SERS），其中使用贵金属纳米颗粒来增强拉曼散射。由于其低毒性和生物相容性，金纳米颗粒（AuNPs）在单细胞分析中通常被优先用作SERS底物。AuNPs的细胞内摄取、定位和毒性问题是解释数据的关键点，因为获得的SERS信号源自细胞摄取的与AuNPs紧邻的分子。在这篇综述中，讨论了AuNP与活细胞的相互作用、AuNPs的细胞摄取和毒性与其物理化学性质的关系，以及单细胞的表面增强拉曼散射。
