Janwan Penchom, Intapan Pewpan M, Yamasaki Hiroshi, Laummaunwai Porntip, Sawanyawisuth Kittisak, Wongkham Chaisiri, Tayapiwatana Chatchai, Kitkhuandee Amnat, Lulitanond Viraphong, Nawa Yukifumi, Maleewong Wanchai
Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. ; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
Korean J Parasitol. 2013 Dec;51(6):751-4. doi: 10.3347/kjp.2013.51.6.751. Epub 2013 Dec 31.
Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.
颚口线虫病是人类颚口线虫病的一种严重形式，可导致疾病甚至死亡。颚口线虫病的诊断主要依据临床表现进行推测。免疫印迹法可识别棘颚口线虫幼虫提取物中分子量为21 kDa和24 kDa的抗原成分（Gs 21/24），对颚口线虫病的诊断具有高灵敏度和特异性。然而，从自然感染或实验动物体内获取的寄生虫中，只能制备出极少量的Gs 21/24抗原。为解决这一问题，我们最近从棘颚口线虫中制备了一种重组基质金属蛋白酶（rMMP）蛋白。在本研究中，我们将这种rMMP与Gs 21/24抗原一起用于人类颚口线虫病的血清学诊断评估。我们研究了泰国孔敬大学诗里那琳医院40例临床症状符合颚口线虫病的患者血清，以及30例泰国健康对照成年人的血清。使用免疫印迹分析对所有血清进行检测，以确定其针对棘颚口线虫粗幼虫提取物和rMMP蛋白的特异性IgG抗体。两种抗原制剂的灵敏度和特异性均为100%。这些结果表明，棘颚口线虫rMMP蛋白可作为幼虫提取物的替代诊断抗原，用于颚口线虫病的血清学诊断。
