Whitham R H, Nilaver G, Bourdette D N, Seil F J
Neurology Research, Veterans Administration Medical Center, Portland, OR 97207.
J Neuroimmunol. 1988 May;18(2):155-70. doi: 10.1016/0165-5728(88)90063-x.
To investigate the role of anti-myelin antibodies in chronic relapsing experimental allergic encephalomyelitis (CR-EAE), sera from SJL/J mice with CR-EAE actively induced by inoculation with spinal cord homogenate in complete Freund's adjuvant (CFA) were compared with sera from mice to whom CR-EAE was passively transferred by lymph node cells (LNC) stimulated with myelin basic protein (BP). Sera were obtained serially from mice during both remissions and relapses of disease and were evaluated for the presence of anti-myelin antibodies using an avidin-biotin-immunoperoxidase technique. Four of six mice with CR-EAE induced with cord-CFA were positive for anti-myelin antibodies 15-124 days after inoculation, with 16 of 18 sera positive in these four mice. Two mice inoculated with cord-CFA did not have detectable serum anti-myelin antibodies, despite a clinical and histopathological picture indistinguishable from the antibody-positive mice. None of seven mice with CR-EAE passively transferred by BP-stimulated LNC had detectable anti-myelin antibodies in 30 sera obtained 7-141 days after cell transfer. We conclude that serum anti-myelin antibodies probably do not play a significant role in the pathogenesis of CR-EAE in SJL/J mice.
为研究抗髓磷脂抗体在慢性复发性实验性变应性脑脊髓炎（CR-EAE）中的作用，将用完全弗氏佐剂（CFA）接种脊髓匀浆主动诱导CR-EAE的SJL/J小鼠血清，与用髓磷脂碱性蛋白（BP）刺激的淋巴结细胞（LNC）被动转移CR-EAE的小鼠血清进行比较。在疾病缓解期和复发期从小鼠连续采集血清，并使用抗生物素蛋白-生物素-免疫过氧化物酶技术评估抗髓磷脂抗体的存在情况。用脊髓-CFA诱导CR-EAE的6只小鼠中有4只在接种后15 - 124天抗髓磷脂抗体呈阳性，这4只小鼠的18份血清中有16份呈阳性。另外2只接种脊髓-CFA的小鼠，尽管临床和组织病理学表现与抗体阳性小鼠无差异，但血清中未检测到抗髓磷脂抗体。在细胞转移后7 - 141天获得的30份血清中，7只经BP刺激的LNC被动转移CR-EAE的小鼠均未检测到抗髓磷脂抗体。我们得出结论，血清抗髓磷脂抗体可能在SJL/J小鼠CR-EAE的发病机制中不发挥重要作用。
