Fu Nan, Wang Ping-Yong, Liu Xiao-Dan, Shen Huo-Lin
College of Agronomy and Biotechnology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian District, Beijing 100193, China.
Molecules. 2014 Feb 10;19(2):1939-55. doi: 10.3390/molecules19021939.
Celery (Apium graveolens L.) is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting celery molecular breeding are quite limited, thus few studies on celery have been conducted so far. In this study we made use of simple sequence repeat (SSR) markers generated from previous celery transcriptome sequencing and attempted to detect the genetic diversity and relationships of commonly used celery accessions and explore the efficiency of the primers used for cultivars identification. Analysis of molecular variance (AMOVA) of Apium graveolens L. var. dulce showed that approximately 43% of genetic diversity was within accessions, 45% among accessions, and 22% among horticultural types. The neighbor-joining tree generated by unweighted pair group method with arithmetic mean (UPGMA), and population structure analysis, as well as principal components analysis (PCA), separated the cultivars into clusters corresponding to the geographical areas where they originated. Genetic distance analysis suggested that genetic variation within Apium graveolens was quite limited. Genotypic diversity showed any combinations of 55 genic SSRs were able to distinguish the genotypes of all 30 accessions.
芹菜（Apium graveolens L.）是全球经济价值最重要的蔬菜之一，但支持芹菜分子育种的遗传和基因组资源相当有限，因此到目前为止对芹菜的研究很少。在本研究中，我们利用先前芹菜转录组测序产生的简单序列重复（SSR）标记，试图检测常用芹菜种质的遗传多样性和关系，并探索用于品种鉴定的引物的效率。对Apium graveolens L. var. dulce的分子方差分析（AMOVA）表明，约43%的遗传多样性存在于种质内，45%存在于种质间，22%存在于园艺类型间。通过非加权算术平均法（UPGMA）生成的邻接树、群体结构分析以及主成分分析（PCA），将品种分为与它们起源的地理区域相对应的簇。遗传距离分析表明，Apium graveolens内的遗传变异相当有限。基因型多样性表明，55个基因SSR的任何组合都能够区分所有30个种质的基因型。
