Kanje M, Lundborg G, Edström A
Department of Zoophysiology, University of Lund, Sweden.
Brain Res. 1988 Jan 26;439(1-2):116-21. doi: 10.1016/0006-8993(88)91467-9.
An in vivo technique which allows local application of drugs to the regenerating rat sciatic nerve for several days is presented. The sciatic nerve was transected at the knee level and a crush lesion was made proximal to the transection. The crush lesion and the nerve segment distal to it was enclosed in a chamber made of silicone tubing (STC). The STC was perfused via a catheter connected to a miniosmotic pump. Regeneration was evaluated with the 'pinch-test' at 3, 4 and 6 days after the enclosure of the nerve. The rate of regeneration in the STC-surrounded nerve segment was 3.5 mm/day after an initial delay of 1.6 days which are values similar to those in uncovered nerves with crush lesions. Perfusion of the STC with either vinblastine, cycloheximide, actinomycin D or mitomycin C inhibited regeneration. The effects were confined to the STC-covered region. Leakage of drugs was too small to affect the nerve outside the chamber. The results suggest that regeneration requires proliferation and protein synthesis in the cells surrounding the growing axons. This technique could be useful for studies of the local effects of various drugs, specific antibodies, potential growth factors etc. on regeneration of peripheral nerves.
本文介绍了一种体内技术，该技术可在数天内将药物局部应用于再生的大鼠坐骨神经。在膝关节水平切断坐骨神经，并在切断部位近端制造挤压损伤。挤压损伤及其远端的神经段被包裹在由硅胶管制成的腔室（STC）中。通过连接到微型渗透泵的导管对STC进行灌注。在神经被包裹后的第3、4和6天，用“捏压试验”评估再生情况。在最初延迟1.6天后，STC包围的神经段的再生速度为每天3.5毫米，这些值与有挤压损伤的未覆盖神经中的值相似。用长春碱、放线菌酮、放线菌素D或丝裂霉素C灌注STC会抑制再生。这些作用局限于STC覆盖的区域。药物泄漏量太小，不会影响腔室外的神经。结果表明，再生需要生长轴突周围细胞的增殖和蛋白质合成。该技术可用于研究各种药物、特异性抗体、潜在生长因子等对周围神经再生的局部作用。
