Widerberg A, Bergman S, Danielsen N, Lundborg G, Dahlin L B
Department of Hand Surgery, University Hospital, Lund University, Malmö, Sweden.
Occup Environ Med. 1997 May;54(5):312-5. doi: 10.1136/oem.54.5.312.
Exposing a hind leg of a rat to vibration induces an injury to the sciatic nerve--a so called conditioning lesion. After such injury induced by vibration the regenerative capacity of the nerve is improved and can be detected as an increased axonal outgrowth from a test crush lesion to the same nerve. The purpose was to study whether the effect of a conditioning lesion induced by vibration can be prevented by local treatment with a Ca2+ channel blocker D600.
D600 (methoxyverapamil) or Ringer's solution was locally applied to the sciatic nerve on one side through a silicone tube connected to a miniosmotic pump, which was implanted subcutaneously. During the same period the hind leg was exposed to vibration (80 Hz; 32 m/s2 root mean squared) for five hours daily for five consecutive days. The other hind leg was not vibrated. After the end of exposure to vibration the sciatic nerves were crushed bilaterally (test crush lesions) and three or six days later the regeneration distances of sensory axons were measured by the pinch reflex test.
Nerves in the control animals (without implanted miniosmotic pumps and nerves on to which Ringer's solution was locally applied) that were exposed to vibration showed a significantly increased outgrowth length of sensory axons from the test crush lesion compared with the non-vibrated side. Such an effect of a conditioning lesion from the exposure to vibration was suppressed by local application of D600.
Local administration of a Ca2+ channel blocker D600 can prevent the effect of a conditioning lesion-that is, the nerve injury induced by vibration can be inhibited by D600. This may have implications for the treatment of patients with neuropathy of the hand induced by vibration.
将大鼠的一条后腿暴露于振动环境会导致坐骨神经损伤——即所谓的预处理损伤。在由振动引起这种损伤后，神经的再生能力会提高，并且可以通过从测试挤压损伤到同一条神经的轴突生长增加来检测到。目的是研究是否可以通过用钙通道阻滞剂D600进行局部治疗来预防由振动引起的预处理损伤的效果。
通过连接到皮下植入的微型渗透泵的硅胶管，将D600（甲氧基维拉帕米）或林格氏液局部应用于一侧的坐骨神经。在此期间，将后腿每天暴露于振动（80赫兹；均方根32米/秒²）5小时，连续5天。另一条后腿不进行振动处理。在振动暴露结束后，双侧挤压坐骨神经（测试挤压损伤），3天或6天后通过捏反射试验测量感觉轴突的再生距离。
与未振动的一侧相比，暴露于振动的对照动物（未植入微型渗透泵且局部应用林格氏液的神经）中，来自测试挤压损伤的感觉轴突的生长长度显著增加。局部应用D600可抑制由振动暴露引起的预处理损伤的这种效果。
局部施用钙通道阻滞剂D600可预防预处理损伤的效果——也就是说，D600可抑制由振动引起的神经损伤。这可能对手部振动诱发神经病患者的治疗具有启示意义。
