Kanje M, Skottner A, Lundborg G, Sjöberg J
Department of Animal Physiology, University of Lund, Sweden.
Brain Res. 1991 Nov 1;563(1-2):285-7. doi: 10.1016/0006-8993(91)91547-e.
Regeneration was measured after the infliction of a crush lesion on rat sciatic nerves which 4 days earlier had been subjected to a distal conditioning transection. Such nerves exhibited an increased outgrowth of nerve fibers as compared to nerves subjected to a single crush lesion. This increased outgrowth could be prevented, if the nerve was locally perfused around the site of the transection during the 4 days conditioning interval, with cycloheximide, actinomycin D and vinblastine, inhibitors of protein-, RNA-synthesis and retrograde axonal transport, respectively. The inhibitory effect of cycloheximide could be overcome by simultaneous perfusion with insulin-like growth factor I (IGF-1). The results suggest that proteins including IGF-1 which are synthesised locally around a nerve lesion and then transported retrogradely could trigger regenerative events in the neuronal cell body.
在对大鼠坐骨神经造成挤压损伤后测量再生情况，这些坐骨神经在4天前已进行远端条件性横断。与仅遭受单次挤压损伤的神经相比，此类神经显示出神经纤维生长增加。如果在4天的条件化间隔期间，分别用蛋白质合成抑制剂环己酰亚胺、RNA合成抑制剂放线菌素D和逆行轴突运输抑制剂长春碱在横断部位周围对神经进行局部灌注，这种生长增加就可以被阻止。同时用胰岛素样生长因子I（IGF-1）灌注可克服环己酰亚胺的抑制作用。结果表明，包括IGF-1在内的在神经损伤部位周围局部合成然后逆行运输的蛋白质可能触发神经元细胞体中的再生事件。
