Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University , Harbin, Heilongjiang 150001, P. R. China.
ACS Appl Mater Interfaces. 2014 Mar 12;6(5):3250-62. doi: 10.1021/am500325w. Epub 2014 Feb 24.
A facile process for the preparation of multifunctional nanospheres combining several advantages of mesoporous channels, up-conversion (UC) luminescence, and photothermal responses into one single entity is reported. First, Gd2O3:Yb/Er assembled mesoporous silica with 2D hexagonal (MCM-41) and 3D cubic (MCM-48) network have been prepared via a one-step procedure. Then, gold nanocrystals with diameter of 5 nm are integrated with the amino group functionalized nanocomposites. Upon 980 nm near infrared (NIR) laser irradiation, a wavelength-dependent enhancement of the UC intensities is observed due to the surface plasmon resonance (SPR) effect of attached gold nanoparticles. These composites have good biocompatibility and sustained anticancer drug (doxorubicin, DOX) release properties, making it a promising candidate for drug delivery. Particularly, under 980 nm NIR laser irradiation, the green UC emission overlaps the SPR band of gold nanocrystals, which causes a photothermal effect of gold nanocrystals and induces a rapid DOX release from the Au hybrid materials. This DOX loaded multifunctional system has an obvious cytotoxic effect and photothermally killing enhanced effect on SKOV3 ovarian cancer cells. The endocytosis process was also demonstrated through confocal laser scanning microscope (CLSM) images. Such novel multifunctional anticancer drug delivery systems, which combine hyperthermia with the chemotherapeutic drugs by synergistic effect, should be of high potential in cancer therapy.
报道了一种将介孔通道、上转换发光和光热响应的多种优势结合在一个单一实体中的多功能纳米球的简便制备方法。首先，通过一步法制备了具有二维六方（MCM-41）和三维立方（MCM-48）网络的 Gd2O3:Yb/Er 组装介孔硅。然后，将直径为 5nm 的金纳米晶与氨基功能化的纳米复合材料结合。在 980nm 近红外（NIR）激光照射下，由于附着的金纳米粒子的表面等离子体共振（SPR）效应，观察到 UC 强度的波长依赖性增强。这些复合材料具有良好的生物相容性和持续的抗癌药物（阿霉素，DOX）释放性能，使其成为一种有前途的药物输送候选物。特别是在 980nm NIR 激光照射下，绿色 UC 发射与金纳米晶的 SPR 带重叠，导致金纳米晶的光热效应，并从 Au 杂化材料中快速释放 DOX。这种负载 DOX 的多功能系统对 SKOV3 卵巢癌细胞具有明显的细胞毒性作用和光热杀伤增强作用。还通过共聚焦激光扫描显微镜（CLSM）图像证明了内吞过程。这种新型的多功能抗癌药物输送系统通过协同作用将热疗与化疗药物结合在一起，在癌症治疗中具有很高的潜力。
