Yu Yibin, Huang Yida, Feng Wanqian, Yang Mei, Shao Baiqi, Li Jingjing, Ye Fangfu
Wenzhou Institute, University of the Chinese Academy of Sciences Wenzhou 325001 China
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health) Wenzhou Zhejiang 325001 China.
RSC Adv. 2021 Aug 31;11(46):29065-29072. doi: 10.1039/d1ra04431a. eCollection 2021 Aug 23.
Posterior segment ocular diseases are highly prevalent worldwide due to the lack of suitable noninvasive diagnostic and therapeutic tactics. Herein, concerning this predicament, we designed a hybrid retina-targeted photothermal theranostic nanoplatform (UCNPs@Bi@SiO@GE HP-lips), based on the unique upconversion luminescence (UCL) imaging of upconversion nanoparticles (UCNPs), efficient photothermal conversion ability of Bi nanoparticles, and thermal-induced phase transition properties of the liposomes (lips). The nanoplatform was functionalized with penetratin (PNT) and hyaluronic acid (HA), to obtain retina-targeted liposomes (HP-lips). Lipophilic genistein (GE) was entrapped into the liposomes (GE HP-lips). An release study showed NIR irradiation could photothermally trigger controlled release of GE from the liposomal platform. Moreover, cellular uptake evaluation UCL imaging demonstrated UCNPs@Bi@SiO@GE HP-lips represented the brightest UCL, compared with other formulations, which is beneficial for the accurate evaluation of the prognosis and severity of angiogenesis-related posterior segment disorders. Therefore, UCNPs@Bi@SiO@GE HP-lips exhibit promising potential as a theranostic nanoplatform for posterior segment ocular diseases.
由于缺乏合适的非侵入性诊断和治疗策略，后段眼部疾病在全球范围内高度流行。在此，针对这一困境，我们基于上转换纳米粒子（UCNPs）独特的上转换发光（UCL）成像、铋纳米粒子的高效光热转换能力以及脂质体（lips）的热诱导相变特性，设计了一种混合的视网膜靶向光热诊疗纳米平台（UCNPs@Bi@SiO@GE HP-lips）。该纳米平台用穿膜肽（PNT）和透明质酸（HA）进行功能化，以获得视网膜靶向脂质体（HP-lips）。亲脂性染料木黄酮（GE）被包裹在脂质体中（GE HP-lips）。一项释放研究表明，近红外（NIR）照射可通过光热作用触发GE从脂质体平台的控释。此外，细胞摄取评估和UCL成像表明，与其他制剂相比，UCNPs@Bi@SiO@GE HP-lips表现出最亮的UCL，这有利于准确评估与血管生成相关的后段疾病的预后和严重程度。因此，UCNPs@Bi@SiO@GE HP-lips作为后段眼部疾病的诊疗纳米平台具有广阔的应用前景。
