Li Jian-Long, Liu Min, Hu Xue-Yi
a State Key Laboratory of Marine Environmental Science , College of Ocean and Earth Sciences, Xiamen University , Xiamen , Fujian , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):255-6. doi: 10.3109/19401736.2014.883613. Epub 2014 Feb 13.
The complete mitochondrial (mt) genome of the saddleback clownfish Amphiprion polymnus was obtained in this study. The circular mtDNA molecule was 16,804 bp in size and the overall nucleotide composition of the H-strand was 29.59% A, 25.93% T, 15.44% G and 29.04% C, with an A + T bias. The complete mitogenome encoded 13 protein-coding genes, 2 rRNAs, 22 tRNAs and 1 control region (D-loop), with the gene arrangement and translation direction basically identical to other typical vertebrate mitogenomes. We found A. polymnus (KJ101554) and A. bicinctus (JQ030887) had the same length in the protein-coding gene ND5 with 1869 bp, while the ND5 in A. ocellaris (AP006017) was 3 bp less than that of A. polymnus and A. bicinctus. Both structures of ND5, however, could translate to amino acid successfully.
本研究获得了鞍背小丑鱼（Amphiprion polymnus）的完整线粒体（mt）基因组。环状线粒体DNA分子大小为16,804 bp，重链的总体核苷酸组成为29.59% A、25.93% T、15.44% G和29.04% C，具有A+T偏向性。完整的线粒体基因组编码13个蛋白质编码基因、2个rRNA、22个tRNA和1个控制区（D-loop），基因排列和翻译方向与其他典型脊椎动物线粒体基因组基本相同。我们发现鞍背小丑鱼（KJ101554）和双带小丑鱼（JQ030887）的蛋白质编码基因ND5长度相同，为1869 bp，而眼斑小丑鱼（AP006017）的ND5比鞍背小丑鱼和双带小丑鱼少3 bp。然而，两种ND5结构都能成功翻译成氨基酸。
