Ye Le, Hu Jing, Wu Kaichang, Wang Yu, Li Jianlong
a South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , China .
b Key Laboratory of South China Sea Fishery Resource Exploitation & Utilization , Ministry of Agriculture , Guangzhou , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1131-2. doi: 10.3109/19401736.2014.933334. Epub 2014 Jul 1.
The complete mitochondrial (mt) genome of the tomato clownfish Amphiprion frenatus was obtained in this study. The circular mtDNA molecule was 16,774 bp in size and the overall nucleotide composition of the H-strand was 29.72% A, 25.81% T, 15.38% G and 29.09% C, with an A + T bias. The complete mitogenome encoded 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a control region (D-loop), with the gene arrangement and translation direction basically identical to other typical vertebrate mitogenomes. The D-loop included termination associated sequence (TAS), central conserved domain (CCD) and conserved sequence block (CSB), and was composed of 6 complete continuity tandem repeat units and an imperfect tandem repeat unit.
本研究获得了番茄小丑鱼(Amphiprion frenatus)的完整线粒体(mt)基因组。环状线粒体DNA分子大小为16,774 bp,重链的总体核苷酸组成为29.72%的A、25.81%的T、15.38%的G和29.09%的C,具有A+T偏向性。完整的线粒体基因组编码13个蛋白质编码基因、2个rRNA、22个tRNA和一个控制区(D环),其基因排列和翻译方向与其他典型脊椎动物线粒体基因组基本相同。D环包括终止相关序列(TAS)、中央保守结构域(CCD)和保守序列块(CSB),由6个完整连续的串联重复单元和一个不完美的串联重复单元组成。