Tao Yong, Li Jian-Long, Liu Min, Hu Xue-Yi
a College of Ocean and Earth Sciences, Xiamen University , Xiamen , Fujian , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):324-5. doi: 10.3109/19401736.2014.892099. Epub 2014 Mar 11.
In this study we determined the complete mitochondrial (mt) genome of the orange clownfish Amphiprion percula. The circular mtDNA molecule was 16,645 bp in size and the overall nucleotide composition of the H-strand was 29.20% A, 25.80% T, 16.03% G and 28.98% C, with an A + T bias. The complete mitogenome encoded 13 protein-coding genes, 2 rRNAs, 22 tRNAs and 1 control region (D-loop), with the gene arrangement and translation direction basically identical to other typical vertebrate mitogenomes. The similarity of the complete mitogenomes between A. percula and A. ocellaris (AP006017) was 95.60%, clearly different at molecular level.
在本研究中,我们测定了橙色小丑鱼(眼斑双锯鱼)的完整线粒体(mt)基因组。环状线粒体DNA分子大小为16,645 bp,重链的总体核苷酸组成为29.20%A、25.80%T、16.03%G和28.98%C,具有A+T偏向性。完整的线粒体基因组编码13个蛋白质编码基因、2个rRNA、22个tRNA和1个控制区(D环),其基因排列和翻译方向与其他典型脊椎动物线粒体基因组基本相同。眼斑双锯鱼和公子小丑鱼(AP006017)的完整线粒体基因组相似性为95.60%,在分子水平上明显不同。