通过线粒体rRNA基因分析进行物种鉴定。

Species identification through mitochondrial rRNA genetic analysis.

作者信息

Yang Li, Tan Zongqing, Wang Daren, Xue Ling, Guan Min-Xin, Huang Taosheng, Li Ronghua

机构信息

1] Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229 [2] Department of Molecular & Cellular Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267.

Division of Human Genetics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229.

出版信息

Sci Rep. 2014 Feb 13;4:4089. doi: 10.1038/srep04089.

Inter-species and intraspecific variations in mitochondrial DNA (mtDNA) were observed in a bioinformatics analysis of the mitochondrial genomic sequences of 11 animal species. Some highly conserved regions were identified in the mitochondrial 12S and 16S ribosomal RNA (rRNA) genes of these species. To test whether these sequences are universally conserved, primers were designed to target the conserved regions of these two genes and were used to amplify DNA from 21 animal tissues, including two of unknown origin. By sequencing these PCR amplicons and aligning the sequences to a database of non-redundant nucleotide sequences, it was confirmed that these amplicons aligned specifically to mtDNA sequences from the expected species of origin. This molecular technique, when combined with bioinformatics, provides a reliable method for the taxonomic classification of animal tissues.

在对11种动物物种的线粒体基因组序列进行生物信息学分析时，观察到线粒体DNA（mtDNA）的种间和种内变异。在这些物种的线粒体12S和16S核糖体RNA（rRNA）基因中鉴定出了一些高度保守的区域。为了测试这些序列是否普遍保守，设计了引物靶向这两个基因的保守区域，并用于扩增来自21种动物组织的DNA，其中包括两种来源不明的组织。通过对这些PCR扩增子进行测序并将序列与非冗余核苷酸序列数据库进行比对，证实这些扩增子与预期来源物种的mtDNA序列特异性比对。这种分子技术与生物信息学相结合，为动物组织的分类学分类提供了一种可靠的方法。