The Institute for Cancer Research, Philadelphia, Pennsylvania.
Planta. 1968 Dec;79(4):292-8. doi: 10.1007/BF00386912.
Auxin and auxin-inhibitors from acidic ether extracts of normal stem tissue of Nicotiana longiflora, N. debneyi-tabacum, their tumor producing F 1 hybrid and non-tumor and tumor-prone segregants were separated by thin-layer chromatography and measured by an Avena curvature test. A significantly higher amount of auxin, very likely IAA, was found in the F 1 hybrid and the tumor-prone segregants as compared to the non-tumor tissues. Inhibitory substances appeared at different Rf's but generally in low amounts. One inhibitor seems to be identical with the "inhibitor β". In general, the results indicate that higher levels of auxin are associated with tumor-prone tissues in the F 1 hybrid and the segregants of a later generation carrying an alien longiflora chromosome on a debneyi-tabacum background. The role of this growth-regulating substance as related to tumor formation in Nicotiana is discussed.
用酸性乙醚提取物从正常的 Nicotiana longiflora、N. debneyi-tabacum 茎组织、其产生肿瘤的 F1 杂种以及非肿瘤和易患肿瘤的分离物中分离出的生长素和生长素抑制剂，并用燕麦曲率试验进行了测量。与非肿瘤组织相比，F1 杂种和易患肿瘤的分离物中发现了明显更高数量的生长素，很可能是 IAA。抑制物质在不同的 Rf 值处出现，但通常数量较少。一种抑制剂似乎与“抑制剂β”相同。总的来说，这些结果表明，较高水平的生长素与 F1 杂种和携带外源长柄烟草染色体的后代分离物中的易患肿瘤组织有关，该染色体位于 debneyi-tabacum 背景上。讨论了这种生长调节剂与烟草肿瘤形成的关系。
