Dargeviciute A, Roux C, Decreux A, Sitbon F, Perrot-Rechenmann C
Institut des Sciences végétales, CNRS, Gif sur Yvette, France.
Plant Cell Physiol. 1998 Oct;39(10):993-1002. doi: 10.1093/oxfordjournals.pcp.a029311.
Early auxin-regulated tobacco cDNAs, belonging to the Aux/IAA gene family have been isolated by screening of a cDNA library prepared from auxin-treated suspension-grown etiolated seedlings of Nicotiana tabacum. The probes used were either RT-PCR fragments or an insert resulting from mRNA differential display selection. All of them possessed the structural features which characterize the Aux/IAA gene products. The auxin response of three distinct Nt-iaa subclasses has been characterized in terms of kinetics, dose-response and specificity as several plant hormones and chemicals have been tested for their ability to alter Nt-iaa mRNA accumulation. Differences of auxin responses have been observed between the Nt-iaa analysed, revealing significant differences of regulation. The effect of the protein synthesis inhibitor cycloheximide suggested that Nt-iaa2.3, Nt-iaa4.3 and strictly related genes can be classified as primary auxin-responsive genes and Nt-iaa28 as a late one. The steady-state mRNA level of these Nt-iaa has also been determined in organs of tobacco plants.
通过筛选从生长素处理的烟草悬浮培养黄化幼苗制备的cDNA文库，分离出了属于Aux/IAA基因家族的早期生长素调节烟草cDNA。所用探针为RT-PCR片段或mRNA差异显示选择产生的插入片段。它们都具有Aux/IAA基因产物的结构特征。由于已经测试了几种植物激素和化学物质改变Nt-iaa mRNA积累的能力，因此从动力学、剂量反应和特异性方面对三种不同的Nt-iaa亚类的生长素反应进行了表征。在所分析的Nt-iaa之间观察到生长素反应的差异，揭示了调控的显著差异。蛋白质合成抑制剂环己酰亚胺的作用表明，Nt-iaa2.3、Nt-iaa4.3和密切相关的基因可归类为初级生长素反应基因，而Nt-iaa28为晚期反应基因。还测定了这些Nt-iaa在烟草植株器官中的稳态mRNA水平。
