Kaikkonen J, Kosonen L, Nyyssönen K, Porkkala-Sarataho E, Salonen R, Korpela H, Salonen J T
Research Institute of Public Health and the Department of Community Health and General Practice, University of Kuopio, Finland.
Free Radic Res. 1998 Jul;29(1):85-92. doi: 10.1080/10715769800300101.
To test the effects of combined coenzyme Q10 (Q10) and d-alpha-tocopheryl acetate supplementation on exercise-induced oxidative stress and muscular damage we conducted a double-blind study in 37 moderately trained male marathon runners. These were randomly allocated to receive either an antioxidant cocktail: 90 mg of Q10 and 13.5 mg of d-alpha-tocopheryl acetate daily (18 men) or placebo (19 men) for three weeks before a marathon (42km) run. Just before the run, plasma Q10 was 282% (p < 0.0001) and plasma vitamin E 16% (p < 0.007) higher in the supplemented group, than in the placebo group. Also the proportion of plasma ubiquinol of total Q10, an indication of plasma redox status in vivo, was significantly higher in the supplemented group. Furthermore, the susceptibility of the VLDL + LDL fraction, to copper-induced oxidation, was significantly reduced in the supplemented group, compared to the placebo group. The exercise increased lipid peroxidation significantly in both study groups, as assessed by the elevated proportion LDL of LDL and the increased susceptibility of lipoproteins to copper induced oxidation. However, the supplementation had no effect on lipid peroxidation or on the muscular damage (increase in serum creatine kinase activity or in plasma lactate levels) induced by exhaustive exercise. Plasma ascorbate, Q10, whole blood glutathione and serum uric acid concentrations increased during the exercise, elevating significantly the TRAP value of plasma by 10.3% and the proportion of plasma ubiquinol of total Q10 by 4.9%. These results suggest that even though exercise increases plasma lipid peroxidation, it also elevates the antioxidative capacity of plasma, as assessed by the increased plasma TRAP and the proportion of Q10H2 of total Q10. However, prior supplementation with small doses of Q10 and d-alpha-tocopheryl acetate neither attenuates the oxidation of lipoproteins nor muscular damage induced by exhaustive exercise such as encountered in a marathon run.
为了测试联合补充辅酶Q10（Q10）和d-α-生育酚醋酸酯对运动诱导的氧化应激和肌肉损伤的影响，我们对37名中度训练的男性马拉松运动员进行了一项双盲研究。这些运动员被随机分配，在马拉松（42公里）跑步前3周，每天接受一种抗氧化剂混合物：90毫克Q10和13.5毫克d-α-生育酚醋酸酯（18名男性）或安慰剂（19名男性）。就在跑步前，补充组的血浆Q10比安慰剂组高282%（p < 0.0001），血浆维生素E高16%（p < 0.007）。此外，补充组中血浆泛醇占总Q10的比例（体内血浆氧化还原状态的一个指标）也显著更高。此外，与安慰剂组相比，补充组中极低密度脂蛋白（VLDL）+低密度脂蛋白（LDL）部分对铜诱导氧化的敏感性显著降低。通过升高的低密度脂蛋白中低密度脂蛋白的比例以及脂蛋白对铜诱导氧化的敏感性增加来评估，运动在两个研究组中均显著增加了脂质过氧化。然而，补充剂对脂质过氧化或力竭运动诱导的肌肉损伤（血清肌酸激酶活性或血浆乳酸水平升高）没有影响。运动期间血浆抗坏血酸、Q10、全血谷胱甘肽和血清尿酸浓度增加，使血浆的总抗氧化能力（TRAP）值显著升高10.3%，血浆泛醇占总Q10的比例升高4.9%。这些结果表明，尽管运动增加了血浆脂质过氧化，但通过增加的血浆TRAP和总Q10中Q10H2的比例评估，它也提高了血浆的抗氧化能力。然而，预先补充小剂量的Q10和d-α-生育酚醋酸酯既不能减轻脂蛋白的氧化，也不能减轻马拉松跑步等力竭运动诱导的肌肉损伤。
