Yunker C E, Kocan K M, Norval R A, Burridge M J
University of Florida/USAID/Zimbabwe Heartwater Research Project, Veterinary Research Laboratory, Causeway, Zimbabwe.
Onderstepoort J Vet Res. 1987 Sep;54(3):183-5.
Mallory's phloxine-methylene blue stain was used to differentiate colonies of Cowdria ruminantium in midgut epithelial cells of nymphal Amblyomma hebraeum that had been infected as larvae. Gut tissues were collected from nymphs that had fed on a susceptible sheep and were fixed in formol-saline on the day of repletion. Paraffin sections, 3-4 micron thick, were then stained and this rendered colonies and cell nuclei densely blue against a uniformly pink background of tick tissues. Colonies were easily distinguished from nuclei by their specific morphology. This method of parasite visualization may be adapted to field-collected ticks for rapid detection of C. ruminantium or to assays of susceptibility of tick populations to various strains of the organism.
使用马洛里派洛宁-亚甲蓝染色法，对幼虫期已感染的希伯来花蜱若虫中肠上皮细胞中的反刍兽考德里氏体菌落进行鉴别。从吸食了易感绵羊血液的若虫身上采集肠道组织，并在饱血当天用甲醛生理盐水固定。然后对3-4微米厚的石蜡切片进行染色，结果在蜱组织均匀的粉红色背景衬托下，菌落和细胞核呈现出深蓝色。通过其特殊形态，菌落很容易与细胞核区分开来。这种寄生虫可视化方法可适用于野外采集的蜱，以便快速检测反刍兽考德里氏体，或用于检测蜱种群对该生物体不同菌株的易感性。
