Chaudhuri J, Martin R E, Donahue M J
Department of Biological Sciences, University of North Texas, Denton 76203.
Parasitology. 1988 Feb;96 ( Pt 1):157-70. doi: 10.1017/s0031182000081737.
The metabolites of 5-hydroxytryptamine (5-HT, serotonin) namely, L-tryptophan, 5-hydroxytryptophan, 5-hydroxyindole acetic acid and 5-hydroxytryptophol were measured in perfused tissue and whole worms from adult female Ascaris suum using reversed-phase liquid chromatography with electrochemical detection. The intracellular levels of each metabolite were quantitated in response to several physiological effectors but only L-tryptophan (TRP) caused dose-dependent changes in these metabolites. Serotonin itself could also be absorbed by perfused A. suum muscle and intestinal tissue. When live A. suum were tied at the anterior and posterior regions to restrict TRP absorption by the intestine, TRP was absorbed through the cuticle and converted into 5-HT by the muscle tissue. In united live parasites TRP absorption was observed in both muscle and intestinal tissue. Collectively, the data indicated that 5-HT may be either absorbed directly or synthesized de novo from absorbed TRP in the isolated tissue of A. suum. The 5-HT, in the adult female A. suum, can be synthesized de novo from TRP, or 5-HT can be absorbed from the environment both through the cuticle and by the intestine of living parasites. Data also indicated that there was preferential sequestering of 5-HT and the metabolites of 5-HT in the anterior tissues of the worms.
采用反相液相色谱-电化学检测法，测定了成年雌性猪蛔虫灌注组织和完整虫体中5-羟色胺（5-HT，血清素）的代谢产物，即L-色氨酸、5-羟色氨酸、5-羟吲哚乙酸和5-羟色醇。针对几种生理效应物对每种代谢产物的细胞内水平进行了定量，但只有L-色氨酸（TRP）引起了这些代谢产物的剂量依赖性变化。血清素自身也可被灌注的猪蛔虫肌肉和肠道组织吸收。当活的猪蛔虫在前后部位被结扎以限制肠道对TRP的吸收时，TRP通过角质层被吸收，并被肌肉组织转化为5-HT。在联合的活寄生虫中，在肌肉和肠道组织中均观察到TRP的吸收。总体而言，数据表明5-HT可能直接被吸收，或者在猪蛔虫的分离组织中由吸收的TRP重新合成。在成年雌性猪蛔虫中，5-HT可由TRP重新合成，或者5-HT可通过角质层以及活寄生虫的肠道从环境中吸收。数据还表明，5-HT及其代谢产物在虫体前部组织中存在优先隔离现象。
