Lacković Z, Parenti M, Neff N H
Eur J Pharmacol. 1981 Jan 29;69(3):347-52. doi: 10.1016/0014-2999(81)90481-7.
A simple rapid method for the determination of 5-hydroxytryptophan, serotonin (5-hydroxytryptamine), and 5-hydroxyindoleacetic acid in brain is presented. Brain proteins are precipitated with Zn(OH)2. The indoles in the supernatant are separated by HPLC in less than than 8 min on a reverse phase column and detected electrochemically. As little as 38 fmol of hydroxyindole compound can be detected and quantitated. Because the method is rapid and uncomplicated many samples can be processed in a day.
本文介绍了一种用于测定脑中5-羟色氨酸、血清素（5-羟色胺）和5-羟吲哚乙酸的简单快速方法。脑蛋白用氢氧化锌沉淀。上清液中的吲哚在反相柱上通过高效液相色谱在不到8分钟内分离，并进行电化学检测。低至38飞摩尔的羟吲哚化合物都能被检测和定量。由于该方法快速且操作简单，一天内可处理许多样品。
