Jafarian-Dehkordi Abbas, Pourradi Nasibeh
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2013 Nov 30;2:86. doi: 10.4103/2277-9175.122503. eCollection 2013.
During the last decades there has been great attention paid to aflatoxins. They are highly toxic, immunosuppressive, mutagenic, teratogenic, and carcinogenic compounds. Aflatoxin M1 (AFM1), a hydroxylated metabolite of aflatoxin B1 (AFB1), is formed in the liver and excreted into the breast milk. It is considered to cause certain hygienic risks for infant health. The aim of this study was to evaluate the presence of the AFM1 in the breast milk using AFM1 in milk as a biomarker for exposure to aflatoxin B1 and determine the level of AFM1 contamination in the lactating mothers in Isfahan, Iran.
This study was carried out on 80 lactating women randomly selected from two urban health centers. Mother's milk samples and information on food intake were collected from the participants using structured food-frequency questionnaire. Breast milk samples were tested for AFM1 by a competitive ELISA technique.
Our findings showed that only one sample was contaminated with AFM1 with concentrations of 6.8 ng/L. However, the AFM1 level in this sample was lower than the maximum tolerable limit (25 ng/L) accepted by the European Communities and Codex Alimentarius.
Although the concentration of AFM1 in none of the samples was higher than the acceptable level, the presence of AFM1 in only one of them confirms the need for developing strategies to reduce exposure to aflatoxin in foods and to carry out biological monitoring of aflatoxins as a food quality control measure routinely.
在过去几十年里，黄曲霉毒素备受关注。它们是剧毒、免疫抑制、诱变、致畸和致癌化合物。黄曲霉毒素M1（AFM1）是黄曲霉毒素B1（AFB1）的羟基化代谢产物，在肝脏中形成并排泄到母乳中。它被认为会对婴儿健康造成一定的卫生风险。本研究的目的是使用母乳中的AFM1作为接触黄曲霉毒素B1的生物标志物，评估母乳中AFM1的存在情况，并确定伊朗伊斯法罕哺乳期母亲的AFM1污染水平。
本研究对从两个城市卫生中心随机选取的80名哺乳期妇女进行。使用结构化食物频率问卷从参与者那里收集母乳样本和食物摄入信息。通过竞争性酶联免疫吸附测定技术检测母乳样本中的AFM1。
我们的研究结果表明，只有一个样本被AFM1污染，浓度为6.8纳克/升。然而，该样本中的AFM1水平低于欧洲共同体和食品法典委员会认可的最大耐受限量（25纳克/升）。
虽然没有一个样本中的AFM1浓度高于可接受水平，但只有其中一个样本中存在AFM1，这证实有必要制定战略以减少食品中黄曲霉毒素的暴露，并将黄曲霉毒素的生物监测作为一种食品质量控制措施常规开展。
