Department of Pharmaceutical Analysis, Fujian Medical University , Fuzhou 350004, China.
Anal Chem. 2014 Mar 4;86(5):2711-8. doi: 10.1021/ac404104j. Epub 2014 Feb 20.
A green approach is proposed for in situ growth of porous platinum nanoparticles on graphene oxide (PtNPs/GO). The resulting nanocomposite has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate in the presence of hydrogen peroxide. On the basis of the peroxidase-like activity, we used the PtNPs/GO as a signal transducer to develop a colorimetric assay for the direct detection of cancer cells. By using folic acid as a recognition element, a total of 125 cancer cells (MCF-7) can be distinguished by naked-eye observation. We envision that this nanomaterial could be used as a power tool for a wide range of potential applications in biotechnology and medicine.
提出了一种绿色方法，用于在氧化石墨烯（GO）上原位生长多孔铂纳米粒子（PtNPs/GO）。该纳米复合材料已被证明可作为过氧化物酶模拟物，在存在过氧化氢的情况下可以催化过氧化物酶底物的反应。基于类过氧化物酶活性，我们将 PtNPs/GO 用作信号转导物，开发了用于直接检测癌细胞的比色测定法。通过使用叶酸作为识别元件，通过肉眼观察可以区分总共 125 个癌细胞（MCF-7）。我们设想这种纳米材料可以用作生物技术和医学中广泛应用的有力工具。
