Cvjetko Petra, Zovko Mira, Balen Biljana
Arh Hig Rada Toksikol. 2014 Mar;65(1):1-18. doi: 10.2478/10004-1254-65-2014-2443.
Plants endure a variety of abiotic and biotic stresses, all of which cause major limitations to production. Among abiotic stressors, heavy metal contamination represents a global environmental problem endangering humans, animals, and plants. Exposure to heavy metals has been documented to induce changes in the expression of plant proteins. Proteins are macromolecules directly responsible for most biological processes in a living cell, while protein function is directly influenced by posttranslational modifications, which cannot be identified through genome studies. Therefore, it is necessary to conduct proteomic studies, which enable the elucidation of the presence and role of proteins under specific environmental conditions. This review attempts to present current knowledge on proteomic techniques developed with an aim to detect the response of plant to heavy metal stress. Significant contributions to a better understanding of the complex mechanisms of plant acclimation to metal stress are also discussed.
植物会遭受各种非生物和生物胁迫，所有这些胁迫都会对产量造成重大限制。在非生物胁迫因素中，重金属污染是一个危及人类、动物和植物的全球环境问题。已有文献记载，接触重金属会诱导植物蛋白质表达发生变化。蛋白质是直接负责活细胞中大多数生物过程的大分子，而蛋白质功能直接受翻译后修饰的影响，而翻译后修饰无法通过基因组研究来识别。因此，有必要进行蛋白质组学研究，以阐明特定环境条件下蛋白质的存在和作用。本综述试图介绍为检测植物对重金属胁迫的反应而开发的蛋白质组学技术的现有知识。还讨论了对更好地理解植物适应金属胁迫的复杂机制的重大贡献。
