Cheema Umber, Brown Robert A
UCL Tissue Repair and Engineering Centre, Institute of Orthopaedics, Division of Surgery, University College London , Stanmore Campus, London, United Kingdom .
Adv Wound Care (New Rochelle). 2013 May;2(4):176-184. doi: 10.1089/wound.2012.0392.
To produce biomimetic collagen scaffolds for tissue modeling and as tissue-engineered implants.
Control of collagen fibril material parameters in collagen hydrogel scaffolds by using plastic compression (PC), resulting in direct control of cell proliferation, cell migration, and cell-cell interaction.
We were able to control the density of collagen in such scaffolds from between 0.2% and 30%, and controllably layer the fibrils in the Z-plane. Cell migration was observed in gels where a gradient of collagen density was present. In these gels, cells preferentially migrated toward the collagen-dense areas. Cell proliferation rates were measurably higher in dense collagen gels.
The use of PC to control material properties of collagen hydrogels results in collagen scaffolds that are biomimetic. These collagen gels reproduce the relevant matrix-mechanical environment in which behavior is more representative of that found .
The material properties of native collagen type I gels can be engineered to match those found in tissues to elicit more biomimetic cell behavior.
制备用于组织建模和作为组织工程植入物的仿生胶原蛋白支架。
通过塑性压缩（PC）控制胶原蛋白水凝胶支架中胶原纤维的材料参数，从而直接控制细胞增殖、细胞迁移和细胞间相互作用。
我们能够将此类支架中胶原蛋白的密度控制在0.2%至30%之间，并在Z平面上可控地排列纤维。在存在胶原蛋白密度梯度的凝胶中观察到细胞迁移。在这些凝胶中，细胞优先向胶原蛋白密集区域迁移。在密集胶原蛋白凝胶中，细胞增殖率明显更高。
使用PC控制胶原蛋白水凝胶的材料特性可产生仿生的胶原蛋白支架。这些胶原蛋白凝胶再现了相关的基质力学环境，其行为更能代表所发现的情况。
天然I型胶原蛋白凝胶的材料特性可经设计以匹配组织中的特性，从而引发更仿生的细胞行为。
