Gawronska-Kozak Barbara
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.
Methods Enzymol. 2014;538:1-13. doi: 10.1016/B978-0-12-800280-3.00001-3.
External murine ears collected postmortem, as well as ear punches obtained during standard marking of live animals, are the source of mesenchymal stem cells, termed ear mesenchymal stem cells (EMSC). These cells provide an easily obtainable, primary culture model system for the study of lineage commitment and differentiation. EMSC are capable of differentiating into adipocytes, osteocytes, chondrocytes, and contractile myocytes. Facile adipogenic differentiation of EMSC provides an excellent model for the study of adipogenesis. In this chapter, methods for isolation, culture, and differentiation of EMSC are described.
死后收集的外部小鼠耳朵，以及在活体动物标准标记过程中获得的耳组织打孔样本，是间充质干细胞的来源，称为耳间充质干细胞（EMSC）。这些细胞为谱系定向和分化研究提供了一个易于获取的原代培养模型系统。EMSC能够分化为脂肪细胞、骨细胞、软骨细胞和收缩性肌细胞。EMSC易于向脂肪细胞分化，为脂肪生成研究提供了一个优秀的模型。在本章中，将描述EMSC的分离、培养和分化方法。
