Monfrecola Giuseppe, Lembo Serena, Cantelli Mariateresa, Ciaglia Elena, Scarpato Luigi, Fabbrocini Gabriella, Balato Anna
Department of Clinical Medicine and Surgery, Section of Dermatology, University of Naples Federico II, Italy.
Department of Pharmaceutical and Biomedical Sciences, University of Salerno, Italy.
Biochimie. 2014 Jun;101:252-5. doi: 10.1016/j.biochi.2014.02.001. Epub 2014 Feb 12.
Visible blue light (BL) spectrum ranges from 400 nm to 475 nm, peaking at 420 nm. Various biological effects have been shown to be exerted by visible light (VIS) (wavelengths (λ): 400-700 nm), including erythema, pigmentation and generation of reactive oxygen species. Due to the sequential position along the electromagnetic radiation (EMR) spectrum, BL biological effects could be theoretically compared to the UVA ones (λ: 320-400 nm). In the present study we investigated the effects of BL on differentiation, maturation and cytokine production of monocytes derived dendritic cells (MDDCs), through the irradiation of their precursors. MDDC precursors (CD14(+)cells) were isolated from the blood of healthy donors and subsequently irradiated with increasing doses of BL. Differentiation as well as maturation process was assessed by flow cytometry, analyzing CD1a, CD83 and CD86 positive cells. Moreover, intracytoplasmatic immunofluorescence, in irradiated vs unirradiated derived cells, was performed to evaluate IL-6 and TNF-α production. Our findings have shown that BL treatment of MDDCp: i) did not affect the generation of iDCs, ii) did not interfere with terminal differentiation of MDDCs (from iDCs to mDCs) and iii) decreased IL-6 and TNF-α production by MDDCs in a dose-dependent manner. We concluded that BL is unable to interfere with MDDC differentiation and maturation, whereas it is effective in reducing the production of IL-6 and TNF-α.
可见蓝光（BL）光谱范围为400纳米至475纳米，峰值在420纳米。已表明可见光（VIS，波长（λ）：400 - 700纳米）会产生多种生物学效应，包括红斑、色素沉着和活性氧的生成。由于在电磁辐射（EMR）光谱中的顺序位置，理论上蓝光的生物学效应可与紫外线A（UVA，λ：320 - 400纳米）的效应相比较。在本研究中，我们通过照射单核细胞衍生树突状细胞（MDDCs）的前体细胞，研究了蓝光对其分化、成熟和细胞因子产生的影响。从健康供体的血液中分离出MDDC前体细胞（CD14(+)细胞），随后用递增剂量的蓝光进行照射。通过流式细胞术分析CD1a、CD83和CD86阳性细胞，评估分化以及成熟过程。此外，对照射组和未照射组衍生细胞进行胞内免疫荧光检测，以评估白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的产生。我们的研究结果表明，蓝光处理MDDCp：i）不影响未成熟树突状细胞（iDCs）的生成，ii）不干扰MDDCs的终末分化（从iDCs到成熟树突状细胞（mDCs）），iii）以剂量依赖方式降低MDDCs产生的IL-6和TNF-α。我们得出结论，蓝光无法干扰MDDC的分化和成熟，而在降低IL-6和TNF-α的产生方面是有效的。
