Eriksson C, Rantapää-Dahlqvist S
Department of Clinical Immunology/Clinical Microbiology, Umeå University, Umeå, Sweden
Department of Public Health and Clinical Medicine/Rheumatology, Umeå University, Umeå, Sweden.
Lupus. 2014 Jun;23(7):691-6. doi: 10.1177/0961203314523869. Epub 2014 Feb 14.
A number of cytokines and chemokines were analysed and related to autoantibodies in blood samples pre-dating the onset of symptoms of systemic lupus erythematosus.
Thirty-five patients with systemic lupus erythematosus (American College of Rheumatology criteria) were identified as having donated blood samples, prior to symptom onset, to the Biobank of northern Sweden. Altogether, 140 age- and sex-matched controls were also identified. The concentrations of interferon-α, interleukin-4, interleukin-9, interleukin-10, interferon inducible protein-10 and monocyte chemotactic protein-1 were analysed using multiplex technology and related to autoantibodies (ANA, ENA, anti-dsDNA and anti-histone antibodies) analysed from the same blood sample.
The interferon-γ inducible protein-10 levels were higher in the pre-symptomatic individuals than in controls (p < 0.05) and correlated with interferon-α (p < 0.01). The interferon-γ inducible protein-10 and interferon-α concentrations were significantly increased in individuals positive for autoantibodies: interferon-γ inducible protein-10 for ANA; anti-SSA/Ro and anti-Jo-1 antibodies; interferon-α with anti-SSB/La antibodies. The levels of interleukin-10, interferon-γ inducible protein-10 and monocyte chemotactic protein-1 increased significantly from the pre-symptomatic individuals to after onset of systemic lupus erythematosus.
An increased concentration of interferon-γ inducible protein-10 pre-dated the onset of systemic lupus erythematosus and was related to autoantibodies before the onset of disease. The levels of interferon-γ inducible protein-10 and interferon-α were correlated. These findings support the proposal that the interferon system is important early in the pathogenesis of systemic lupus erythematosus and autoantibody formation.
分析了一些细胞因子和趋化因子，并将其与系统性红斑狼疮症状出现前血样中的自身抗体相关联。
35例符合美国风湿病学会标准的系统性红斑狼疮患者被确定在症状出现前已向瑞典北部生物样本库捐献血样。总共还确定了140名年龄和性别匹配的对照者。使用多重技术分析了干扰素-α、白细胞介素-4、白细胞介素-9、白细胞介素-10、干扰素诱导蛋白-10和单核细胞趋化蛋白-1的浓度，并将其与从同一血样中分析的自身抗体（抗核抗体、可提取核抗原、抗双链DNA和抗组蛋白抗体）相关联。
症状出现前个体的干扰素-γ诱导蛋白-10水平高于对照组（p<0.05），且与干扰素-α相关（p<0.01）。自身抗体阳性个体中，干扰素-γ诱导蛋白-10和干扰素-α浓度显著升高：抗核抗体、抗SSA/Ro和抗Jo-1抗体对应的干扰素-γ诱导蛋白-10；抗SSB/La抗体对应的干扰素-α。从症状出现前个体到系统性红斑狼疮发病后，白细胞介素-10、干扰素-γ诱导蛋白-10和单核细胞趋化蛋白-1水平显著升高。
干扰素-γ诱导蛋白-10浓度升高早于系统性红斑狼疮发病，且与疾病发作前的自身抗体有关。干扰素-γ诱导蛋白-10和干扰素-α水平相关。这些发现支持了干扰素系统在系统性红斑狼疮发病机制和自身抗体形成早期很重要的观点。
