Narayana Sai Sathya, Deepa Vinoth Kumar, Ahamed Shafie, Sathish Emmanuel Solomon, Meyappan R, Satheesh Kumar K S
Department of Endodontics, Rajah Muthiah Dental College and Hospital, Annamalai University, Annamalai Nagar, Tamil Nadu, India.
J Indian Soc Pedod Prev Dent. 2014 Jan-Mar;32(1):19-25. doi: 10.4103/0970-4388.127047.
The objective of this study is to investigate the efficacy of bioactive glass containing product on remineralization of artificial induced carious enamel lesion and to compare its efficiency with other remineralization products using an in-vitro pH cycling method. The null hypothesis tested was bioactive glass has no effect on enamel remineralization.
A total of 20 enamel samples of human molar teeth were subjected to artificial caries lesion formation using pH cycling method and was verified using high resolution scanning electron microscope (HRSEM). Each demineralized sample was then divided into five test groups each containing twenty. Group A - Bioactive glass (SHY-NM), Group B - Fluoride tooth paste (Amflor), Group C - CPP-ACP (Tooth mousse), Group D - CPP-ACPF (Tooth mousse plus), Group E - control. All the test groups were exposed to the pH cycling regime, the remineralizing agents were applied for 10 min except control. After 10 days period, the entire test groups were evaluated with HRSEM and quantitative assessment by energy dispersive X-ray spectroscopy. The obtained data was analyzed statistically using one-way ANOVA, Student's t-test and Tukey's multiple comparison tests. P ≤ 0.05 was considered to be significant.
Rejection of the null hypothesis and highlights the concept of biomimetic bioactive glass as an effective remineralizing agent.
To focus on the importance of minimal invasive treatment on incipient carious lesion by remineralization.
本研究的目的是使用体外pH循环法研究含生物活性玻璃产品对人工诱导龋损牙釉质再矿化的疗效，并将其效率与其他再矿化产品进行比较。所检验的无效假设是生物活性玻璃对牙釉质再矿化没有影响。
使用pH循环法对20颗人磨牙牙釉质样本进行人工龋损形成，并使用高分辨率扫描电子显微镜（HRSEM）进行验证。然后将每个脱矿样本分为五个测试组，每组20个。A组 - 生物活性玻璃（SHY-NM），B组 - 含氟牙膏（Amflor），C组 - 酪蛋白磷酸肽-无定形磷酸钙（Tooth mousse），D组 - 酪蛋白磷酸肽-无定形磷酸钙氟化物（Tooth mousse plus），E组 - 对照组。所有测试组均接受pH循环方案，除对照组外，再矿化剂应用10分钟。10天后，使用HRSEM对整个测试组进行评估，并通过能量色散X射线光谱进行定量评估。使用单因素方差分析、学生t检验和Tukey多重比较检验对获得的数据进行统计分析。P≤0.05被认为具有统计学意义。
无效假设被拒绝，突出了仿生生物活性玻璃作为一种有效再矿化剂的概念。
强调通过再矿化对早期龋损进行微创治疗的重要性。
