Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, California, USA.
J Bacteriol. 2014 May;196(9):1683-93. doi: 10.1128/JB.01258-13. Epub 2014 Feb 14.
In microbiology, gene disruption and subsequent experiments often center on phenotypic changes caused by one class of specialized metabolites (quorum sensors, virulence factors, or natural products), disregarding global downstream metabolic effects. With the recent development of mass spectrometry-based methods and technologies for microbial metabolomics investigations, it is now possible to visualize global production of diverse classes of microbial specialized metabolites simultaneously. Using imaging mass spectrometry (IMS) applied to the analysis of microbiology experiments, we can observe the effects of mutations, knockouts, insertions, and complementation on the interactive metabolome. In this study, a combination of IMS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to visualize the impact on specialized metabolite production of a transposon insertion into a Pseudomonas aeruginosa phenazine biosynthetic gene, phzF2. The disruption of phenazine biosynthesis led to broad changes in specialized metabolite production, including loss of pyoverdine production. This shift in specialized metabolite production significantly alters the metabolic outcome of an interaction with Aspergillus fumigatus by influencing triacetylfusarinine production.
在微生物学中，基因敲除和随后的实验通常集中在一类特殊代谢物（群体感应传感器、毒力因子或天然产物）引起的表型变化上，而忽略了全局下游代谢效应。随着基于质谱的微生物代谢组学研究方法和技术的最新发展，现在可以同时可视化多种微生物特殊代谢物的全局产生。通过将成像质谱 (IMS) 应用于微生物学实验的分析，我们可以观察到突变、敲除、插入和互补对相互代谢组的影响。在这项研究中，组合使用 IMS 和液相色谱-串联质谱 (LC-MS/MS) 来观察转座子插入铜绿假单胞菌吩嗪生物合成基因 phzF2 对特殊代谢产物产生的影响。吩嗪生物合成的破坏导致特殊代谢产物产生的广泛变化，包括丧失绿脓菌素的产生。这种特殊代谢产物产生的转变通过影响三乙酰基麦角新碱的产生，显著改变了与烟曲霉相互作用的代谢结果。
