电离辐射对体外培养的鸡胚胎软骨性胫骨代谢及纵向生长的影响。
Effects of ionizing radiation on the metabolism and longitudinal growth of cartilaginous embryonic chick tibiae in vitro.
作者信息
de Ridder L, Thierens H, Cornelissen M, Segaert O
机构信息
Laboratory for Histology, State University of Gent, Belgium.
出版信息
Int J Radiat Biol Relat Stud Phys Chem Med. 1988 Jun;53(6):965-75. doi: 10.1080/09553008814551321.
The effect of ionizing radiation on the metabolism and longitudinal growth of cartilaginous tibiae of 6.5-day-old chick embryos was studied in vitro over a 3-day period. Before being cultured, tibiae received absorbed doses of 2 to 200 Gy. Of each pair, the counterpart served as control. Compared to the strong inhibition of [3H]thymidine incorporation, already 50 percent at 10 Gy, the effects of ionizing radiation on [3H]uridine and [3H]proline incorporation were limited: 20 and 40 percent respectively at 150 Gy. Metabolism of the cartilage cells in our organ culture was almost completely arrested at 200 Gy. Light and electron microscopy showed no morphological differences between irradiated and sham-irradiated tibiae until 150 Gy. At 200 Gy necrosis of most of the cells was observed. No differences in form and arrangement of extracellular fibers were noticed. The results of the metabolic studies and the morphological observations were correlated with the effects of ionizing radiation on the longitudinal growth. In contrast to DNA synthesis, RNA transcription and synthesis of collagen fibres were radioresistant processes.
在体外对6.5日龄鸡胚软骨胫骨进行了为期3天的研究，以观察电离辐射对其代谢和纵向生长的影响。在培养前，胫骨接受2至200 Gy的吸收剂量。每对胫骨中，另一个作为对照。与[3H]胸苷掺入受到的强烈抑制相比（10 Gy时即达50%），电离辐射对[3H]尿苷和[3H]脯氨酸掺入的影响有限：150 Gy时分别为20%和40%。在200 Gy时，我们器官培养中的软骨细胞代谢几乎完全停止。光学显微镜和电子显微镜显示，在150 Gy之前，辐照胫骨和假辐照胫骨之间没有形态学差异。在200 Gy时观察到大多数细胞坏死。未注意到细胞外纤维的形态和排列有差异。代谢研究结果和形态学观察结果与电离辐射对纵向生长的影响相关。与DNA合成相反，RNA转录和胶原纤维合成是抗辐射过程。
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