Asjö B, Albert J, Chiodi F, Fenyö E M
Department of Virology, Karolinska Institute Stockholm, Sweden.
J Virol Methods. 1988 Mar-Apr;19(3-4):191-6. doi: 10.1016/0166-0934(88)90013-4.
We present an improved culture technique for propagation of human immunodeficiency virus (HIV) strains that grow slowly, yield low reverse transcriptase activity and can be transmitted poorly, if at all, to cell lines. By using the Jurkat-tatIII cell line 29 (of 30) of these slow/low HIV strains, including one West African strain, could be shown to replicate over a period of several weeks.
我们提出了一种改进的培养技术，用于繁殖人类免疫缺陷病毒（HIV）毒株，这些毒株生长缓慢，逆转录酶活性低，并且即便能传播，也很难传播到细胞系中。通过使用Jurkat-tatIII细胞系，在这些生长缓慢/低产的HIV毒株（共30种）中，有29种（包括一种西非毒株）能够在数周时间内进行复制。
