Barsumian E L, Deleon R P, David E, Kinkade P R, Homon C A, Farina P R
Department of Biochemistry, Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT 06877.
Prostaglandins Leukot Essent Fatty Acids. 1988 Mar;31(3):147-53. doi: 10.1016/0952-3278(88)90111-1.
The generation of leukotrienes and histamine release by the mouse mastocytoma cell line MMC-16 was investigated. These cells produced leukotriene C4 (LTC4) and released histamine upon calcium ionophore A23187 and antigen stimulation. The ionophore also stimulated the biosynthesis of leukotriene B4 (LTB4) by MMC-16. Generation of LTC4 was confirmed by its characteristic UV absorption spectrum, fast atom bombardment-MS, equivalent HPLC retention time with an authentic standard and radioimmunoassay. Leukotriene B4 was characterized by its distinctive UV spectrum and HPLC retention time compared with synthetic material. IgE-mediated LTC4 generation was also observed in a dose dependent fashion with MMC-16 cells passively sensitized with monoclonal IgE specific for ovalbumin. LTC4 biosynthesis was effectively inhibited by the lipoxygenase inhibitor NDGA.
