Goetzl E J, Phillips M J, Gold W M
J Exp Med. 1983 Sep 1;158(3):731-7. doi: 10.1084/jem.158.3.731.
Isolated dog mastocytoma cells sensitized with dog anti-ragweed IgE and challenged with ragweed antigen or incubated with ionophore A23187 or the carboxy-terminal dodecapeptide of platelet factor 4, PF4(59-70), release histamine and concurrently generate leukotrienes B4, C4, and D4. In contrast, the exposure of mastocytoma cells to 0.1-3 micrograms/ml of 15-hydroxyeicosatetraenoic acid (15-HETE) stimulates selectively the generation of leukotrienes, in the absence of histamine release, while 0.1-1 micrograms/ml of compound 48/80 releases histamine without enhancing the generation of leukotrienes. That natural stimuli are capable of selectively activating one synthetic or secretory compartment of mast cells suggests that separate subsets of receptors as well as different biochemical events may serve to mobilize each class of mediators.
用犬抗豚草IgE致敏并与豚草抗原接触，或与离子载体A23187或血小板因子4（PF4）的羧基末端十二肽PF4(59 - 70)一起孵育的分离犬肥大细胞瘤细胞，会释放组胺并同时生成白三烯B4、C4和D4。相比之下，肥大细胞瘤细胞暴露于0.1 - 3微克/毫升的15 - 羟基二十碳四烯酸（15 - HETE）中，在不释放组胺的情况下选择性地刺激白三烯的生成，而0.1 - 1微克/毫升的化合物48/80释放组胺但不增强白三烯的生成。天然刺激物能够选择性地激活肥大细胞的一个合成或分泌区室，这表明不同的受体亚群以及不同的生化事件可能参与动员每一类介质。
