Colado Maria Isabel, Del Rio Joaquín, Peralta Enrique
Cajal Institute, CSIC, MadridSpain Department of Pharmacology, Unirersity of Navarra, Medical School, PamplonaSpain.
Pain. 1994 Jan;56(1):3-8. doi: 10.1016/0304-3959(94)90144-9.
In the rat, sciatic and saphenous nerve section resulted in self-mutilation of the ipsilateral limb. Fifteen and 60 days after surgery, monoamine levels were altered not only in the spinal cord but also in supraspinal structures. Thus, in the ipsi- and contralateral sides of the spinal lumbar region, an increase in the levels of 5-hydroxyindoleacetic acid (5-HIAA) was observed 15 days after surgery and in the levels of serotonin (5-HT) and noradrenaline 60 days later. Changes in the content of 5-HT and its metabolite were also evident, at these time points, in periaqueductal gray matter and trigeminal nucleus. Chemical sympathectomy carried out by administering guanethidine to neonatal rats reduced the degree of autotomy and suppressed the changes in monoaminergic systems following peripheral neurectomy. This study supports the hypothesis that the local noradrenaline outflow from sympathetic fibers in the neuroma is one of the causal factors in autotomy and it indicates that autotomy is under the control of descending monoaminergic pathways originating in brain-stem nuclei.
在大鼠中，坐骨神经和隐神经切断导致同侧肢体自残。术后15天和60天，单胺水平不仅在脊髓中发生改变，在脊髓上结构中也发生改变。因此，在脊髓腰段的同侧和对侧，术后15天观察到5-羟吲哚乙酸（5-HIAA）水平升高，60天后观察到血清素（5-HT）和去甲肾上腺素水平升高。在这些时间点，中脑导水管周围灰质和三叉神经核中5-HT及其代谢产物含量的变化也很明显。通过给新生大鼠施用胍乙啶进行化学交感神经切除术降低了自残程度，并抑制了外周神经切除术后单胺能系统的变化。这项研究支持了这样一种假说，即神经瘤中交感神经纤维的局部去甲肾上腺素流出是自残的因果因素之一，并且表明自残受源自脑干核的下行单胺能通路的控制。
