Wang Yu-Ming, Wu Feng-Juan, Du Lei, Li Guo-Yun, Takahashi Koretaro, Xue Yong, Xue Chang-Hu
College of Food Science and Engineering, Ocean University of China, No. 5 Yushan Road, Qingdao, Shandong Province 266003, PR China.
Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan.
Int J Biol Macromol. 2014 May;66:354-61. doi: 10.1016/j.ijbiomac.2014.01.032. Epub 2014 Feb 15.
Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino. The polysaccharides' compositions were analysed, and their effects on HepG2 cell proliferation were assessed. AVAP I had a greater growth-stimulatory effect than AAP or AVAP II. The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity. Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium. AVAP I could therefore promote HepG2 cell proliferation by regulating gene expression and accelerating the cell cycle process. AVAP I may be useful as a serum supplement for stimulating the proliferation of mammalian cells. Our results offer a comprehensive method for utilising the abalone viscera, which is usually discarded as waste.
从皱纹盘鲍（Haliotis discus hannai Ino）中分离出三种多糖，即AAP、AVAP I和AVAP II。对这些多糖的组成进行了分析，并评估了它们对HepG2细胞增殖的影响。AVAP I比AAP或AVAP II具有更强的生长刺激作用。AVAP I的寡糖（Oli-AVAP I）表现出相同的生长效应，但AVAP I和Oli-AVAP I的主要单糖鼠李糖并未表现出这种活性。此外，AVAP I显著降低了CDK6和细胞周期蛋白E1的mRNA水平，但显著增加了细胞周期蛋白B1、CDK1和细胞周期蛋白F。有趣的是，AVAP I在低血清浓度培养基中仍能诱导细胞增殖。因此，AVAP I可通过调节基因表达和加速细胞周期进程来促进HepG2细胞增殖。AVAP I可能作为一种血清补充剂用于刺激哺乳动物细胞的增殖。我们的研究结果提供了一种综合利用通常作为废弃物丢弃的鲍鱼内脏的方法。
