染色质与剪接
Chromatin and splicing.
作者信息
Haque Nazmul, Oberdoerffer Shalini
机构信息
Laboratory of Ribonucleoprotein Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2014;1126:97-113. doi: 10.1007/978-1-62703-980-2_7.
In the past several years, the relationship between chromatin structure and mRNA processing has been the source of significant investigation across diverse disciplines. Central to these efforts was an unanticipated nonrandom distribution of chromatin marks across transcribed regions of protein-coding genes. In addition to the presence of specific histone modifications at the 5' and 3' ends of genes, exonic DNA was demonstrated to present a distinct chromatin landscape relative to intronic DNA. As splicing in higher eukaryotes predominantly occurs co-transcriptionally, these studies raised the possibility that chromatin modifications may aid the spliceosome in the detection of exons amidst vast stretches of noncoding intronic sequences. Recent investigations have supported a direct role for chromatin in splicing regulation and have suggested an intriguing role for splicing in the establishment of chromatin modifications. Here we will summarize an accumulating body of data that begins to reveal extensive coupling between chromatin structure and pre-mRNA splicing.
在过去几年中，染色质结构与mRNA加工之间的关系一直是不同学科大量研究的主题。这些研究的核心是染色质标记在蛋白质编码基因转录区域的意外非随机分布。除了在基因的5'和3'端存在特定的组蛋白修饰外，外显子DNA相对于内含子DNA呈现出独特的染色质景观。由于高等真核生物中的剪接主要在转录过程中发生，这些研究提出了一种可能性，即染色质修饰可能有助于剪接体在大片非编码内含子序列中检测外显子。最近的研究支持了染色质在剪接调控中的直接作用，并暗示了剪接在染色质修饰建立中的有趣作用。在这里，我们将总结一系列不断积累的数据，这些数据开始揭示染色质结构与前体mRNA剪接之间的广泛联系。
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