Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois.
Invest Ophthalmol Vis Sci. 2014 Mar 13;55(3):1588-93. doi: 10.1167/iovs.13-13537.
The purpose of the study is to report global measurements of inner retinal oxygen delivery (DO2_IR) and oxygen metabolism (MO2_IR) in streptozotocin (STZ) diabetic rats.
Phosphorescence lifetime and blood flow imaging were performed in rats 4 (STZ/4 wk; n = 10) and 6 (STZ/6 wk; n = 10) weeks following injection of STZ to measure retinal arterial (O2A) and venous (O2V) oxygen contents and total retinal blood flow (F). DO2_IR and MO2_IR were calculated from measurements of F and O2A and of F and the arteriovenous oxygen content difference, respectively. Data in STZ rats were compared to those in healthy control rats (n = 10).
Measurements of O2A and O2V were not significantly different among STZ/4 wk, STZ/6 wk, and control rats (P ≥ 0.28). Likewise, F was similar among all groups of rats (P = 0.81). DO2_IR measurements were 941 ± 231, 956 ± 232, and 973 ± 243 nL O2/min in control, STZ/4 wk, and STZ/6 wk rats, respectively (P = 0.95). MO2_IR measurements were 516 ± 175, 444 ± 103, and 496 ± 84 nL O2/min in control, STZ/4 wk, and STZ/6 wk rats, respectively (P = 0.37).
Global inner retinal oxygen delivery and metabolism were not significantly impaired in STZ rats in early diabetes.
本研究旨在报告链脲佐菌素（STZ）糖尿病大鼠内视网膜氧输送（DO2_IR）和氧代谢（MO2_IR）的全球测量结果。
在 STZ 注射后 4（STZ/4 周；n = 10）和 6（STZ/6 周；n = 10）周，通过磷光寿命和血流成像测量大鼠视网膜动脉（O2A）和静脉（O2V）氧含量以及总视网膜血流（F）。DO2_IR 和 MO2_IR 分别通过测量 F 和 O2A 以及 F 和动静脉氧含量差来计算。将 STZ 大鼠的数据与健康对照组大鼠（n = 10）的数据进行比较。
STZ/4 周、STZ/6 周和对照组大鼠的 O2A 和 O2V 测量值无显著差异（P ≥ 0.28）。同样，所有大鼠组的 F 也相似（P = 0.81）。对照组、STZ/4 周和 STZ/6 周大鼠的 DO2_IR 测量值分别为 941 ± 231、956 ± 232 和 973 ± 243 nL O2/min（P = 0.95）。对照组、STZ/4 周和 STZ/6 周大鼠的 MO2_IR 测量值分别为 516 ± 175、444 ± 103 和 496 ± 84 nL O2/min（P = 0.37）。
在早期糖尿病中，STZ 大鼠的内视网膜氧输送和代谢没有明显受损。
