链脲佐菌素诱导糖尿病大鼠视网膜内层的氧供与代谢。
Inner retinal oxygen delivery and metabolism in streptozotocin diabetic rats.
机构信息
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois.
出版信息
Invest Ophthalmol Vis Sci. 2014 Mar 13;55(3):1588-93. doi: 10.1167/iovs.13-13537.
PURPOSE
The purpose of the study is to report global measurements of inner retinal oxygen delivery (DO2_IR) and oxygen metabolism (MO2_IR) in streptozotocin (STZ) diabetic rats.
METHODS
Phosphorescence lifetime and blood flow imaging were performed in rats 4 (STZ/4 wk; n = 10) and 6 (STZ/6 wk; n = 10) weeks following injection of STZ to measure retinal arterial (O2A) and venous (O2V) oxygen contents and total retinal blood flow (F). DO2_IR and MO2_IR were calculated from measurements of F and O2A and of F and the arteriovenous oxygen content difference, respectively. Data in STZ rats were compared to those in healthy control rats (n = 10).
RESULTS
Measurements of O2A and O2V were not significantly different among STZ/4 wk, STZ/6 wk, and control rats (P ≥ 0.28). Likewise, F was similar among all groups of rats (P = 0.81). DO2_IR measurements were 941 ± 231, 956 ± 232, and 973 ± 243 nL O2/min in control, STZ/4 wk, and STZ/6 wk rats, respectively (P = 0.95). MO2_IR measurements were 516 ± 175, 444 ± 103, and 496 ± 84 nL O2/min in control, STZ/4 wk, and STZ/6 wk rats, respectively (P = 0.37).
CONCLUSIONS
Global inner retinal oxygen delivery and metabolism were not significantly impaired in STZ rats in early diabetes.
目的
本研究旨在报告链脲佐菌素（STZ）糖尿病大鼠内视网膜氧输送（DO2_IR）和氧代谢（MO2_IR）的全球测量结果。
方法
在 STZ 注射后 4（STZ/4 周；n = 10）和 6（STZ/6 周；n = 10）周，通过磷光寿命和血流成像测量大鼠视网膜动脉（O2A）和静脉（O2V）氧含量以及总视网膜血流（F）。DO2_IR 和 MO2_IR 分别通过测量 F 和 O2A 以及 F 和动静脉氧含量差来计算。将 STZ 大鼠的数据与健康对照组大鼠（n = 10）的数据进行比较。
结果
STZ/4 周、STZ/6 周和对照组大鼠的 O2A 和 O2V 测量值无显著差异（P ≥ 0.28）。同样，所有大鼠组的 F 也相似（P = 0.81）。对照组、STZ/4 周和 STZ/6 周大鼠的 DO2_IR 测量值分别为 941 ± 231、956 ± 232 和 973 ± 243 nL O2/min（P = 0.95）。对照组、STZ/4 周和 STZ/6 周大鼠的 MO2_IR 测量值分别为 516 ± 175、444 ± 103 和 496 ± 84 nL O2/min（P = 0.37）。
结论
在早期糖尿病中，STZ 大鼠的内视网膜氧输送和代谢没有明显受损。
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