de Carvalho Monteiro Juliana Santos, de Oliveira Susana Carla Pires Sampaio, de F Tima Ferreira Lima Maria, Sousa Jos Augusto Cardoso, Pinheiro Ant Nio Luiz Barbosa, Dos Santos Jean Nunes
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Brazil.
Photomed Laser Surg. 2011 Nov;29(11):767-71. doi: 10.1089/pho.2011.3035. Epub 2011 Jul 26.
This article aimed to study the effect of LED Phototherapy (LED-PHT) (?630?nm or ?850?nm) on mast cells on the dorsum of the tongue of rodents.
Vasodilatation is one of the reported effects of laser light on tissues. Laser light is able to induce the release of mediators responsible for vasodilatation, such as those produced by mast cells. Mast cells are also related to some diseases such as hay fever.
Sixty Wistar rats were divided into three groups: I, Control; II, IR-LED (?850?nm, 21.9?J/cm(2), 73 sec; and III, red-LED (?630?nm, 21.9?J/cm(2), 73?sec). The specimens were taken after, 20, 45, and 60?min following irradiation. The specimens were routinely processed; stained with toluidine blue; and then total, degranulated, and non-degranulated mast cells were counted and statistical analysis performed.
Both LED irradiated subjects showed significant difference when compared to the control subjects on the total number mast cells (p<0.001, ANOVA), degranulated mast cells (p<0.001, ANOVA), and non-degranulated mast cells (p<0.001, ANOVA). Comparing the two groups of LED irradiated subjects, significant difference was observed regarding the total number of cells (p<0.001, paired t-test) and degranulated mast cells (p<0.001, paired t-test) with a greater number of these cells noted in the IR-LED group. On the other hand, Red-LED irradiated subjects showed a significantly greater number of non-degranulated mast cells (p=0.001, paired t-test).
Our results lead us to conclude that both red and IR-LED light caused increased mast cell degranulation and that IR-LED light resulted in a greater number of mast cells.
本文旨在研究LED光疗法（波长630nm或850nm）对啮齿动物舌背肥大细胞的影响。
血管舒张是激光对组织的已知作用之一。激光能够诱导负责血管舒张的介质释放，例如肥大细胞产生的介质。肥大细胞也与一些疾病有关，如花粉症。
将60只Wistar大鼠分为三组：I组，对照组；II组，红外LED组（850nm，21.9J/cm²，73秒）；III组，红色LED组（630nm，21.9J/cm²，73秒）。照射后20、45和60分钟采集标本。标本常规处理；用甲苯胺蓝染色；然后对总肥大细胞、脱颗粒肥大细胞和未脱颗粒肥大细胞进行计数并进行统计分析。
与对照组相比，接受LED照射的两组在肥大细胞总数（p<0.001，方差分析）、脱颗粒肥大细胞（p<0.001，方差分析）和未脱颗粒肥大细胞（p<0.001，方差分析）方面均有显著差异。比较两组接受LED照射的大鼠，在细胞总数（p<0.001，配对t检验）和脱颗粒肥大细胞（p<0.001，配对t检验）方面观察到显著差异，红外LED组的这些细胞数量更多。另一方面，红色LED照射的大鼠显示未脱颗粒肥大细胞数量显著更多（p = 0.001，配对t检验）。
我们的结果使我们得出结论，红色和红外LED光均导致肥大细胞脱颗粒增加，并且红外LED光导致更多的肥大细胞。
