Wilcox G L
Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455.
Ann N Y Acad Sci. 1988;525:228-36. doi: 10.1111/j.1749-6632.1988.tb38608.x.
Compounds that produce depolarization of nociceptive neurons in the dorsal horn of the spinal cord also elicit a rather specific kind of caudally directed biting, licking, and/or scratching behavior when they are injected intrathecally in mice. We sought to use this elicited grooming behavior as a test for compounds that might inhibit the neurons excited by the excitatory agents. All three neurokinins--substance P, neurokinin A (substance K), neurokinin B (neuromedin K)--and excitatory amino acids active at N-methyl-D-aspartate (NMDA) or quisqualate receptors produce similar behaviors, which last for 1 minute after i.t. injection. Our data indicate that mu opioid agonists or alpha adrenergic agonists block both neurokinin-elicited behavior and EAA-elicited behavior; delta opioid agonists block only neurokinin-elicited behavior; and PCP/sigma "opioid" agonists block only EAA-elicited behavior. Somatostatin and serotonin produce qualitatively different behaviors by themselves and, when administered with neurokinins, partially block neurokinin-elicited behavior.
当鞘内注射到小鼠体内时，能使脊髓背角伤害性神经元去极化的化合物也会引发一种相当特殊的、向尾侧的咬、舔和/或抓挠行为。我们试图利用这种引发的梳理行为来测试可能抑制由兴奋性物质兴奋的神经元的化合物。所有三种神经激肽——P物质、神经激肽A（物质K）、神经激肽B（神经介素K）——以及对N-甲基-D-天冬氨酸（NMDA）或喹啉酸受体有活性的兴奋性氨基酸都会产生类似的行为，鞘内注射后持续1分钟。我们的数据表明，μ阿片受体激动剂或α肾上腺素能激动剂既能阻断神经激肽引发的行为，也能阻断兴奋性氨基酸引发的行为；δ阿片受体激动剂仅阻断神经激肽引发的行为；而苯环己哌啶/σ“阿片样”激动剂仅阻断兴奋性氨基酸引发的行为。生长抑素和5-羟色胺自身会产生性质不同的行为，当与神经激肽一起给药时，会部分阻断神经激肽引发的行为。
