Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2014 May;64(Pt 5):1736-1742. doi: 10.1099/ijs.0.060848-0. Epub 2014 Feb 19.
A yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070T) was isolated as a presumptive endophyte from internal stem tissue of a healthy corn stem. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain JM-1070T in the monophyletic cluster of the genus Mucilaginibacter, with closest affiliation to the type strains of Mucilaginibacter composti (98% similarity) and Mucilaginibacter calamicampi (97.2%). 16S rRNA gene sequence similarity to type strains of other species of the genus Mucilaginibacter was 93.4-96.0%. DNA-DNA hybridizations between strain JM-1070T and the type strains M. composti LMG 23497T and M. calamicampi CCUG 63418T showed low relatedness values of 13% (reciprocal 18%) and 52% (reciprocal 54.4%). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids (>15%) were iso-C15:0, iso-C15:0 2-OH/C16:1ω7c (measured as summed feature 3) and iso-C17:0 3-OH. Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly sym-homospermidine. Characterization by 16S rRNA gene sequencing, physiological parameters and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain JM-1070T represents a novel species of the genus Mucilaginibacter. For this reason, we propose the name Mucilaginibacter auburnensis sp. nov., with the type strain JM-1070T (=CIP 110694T=LMG 28078T).
一株黄色、革兰氏阴性、杆状、不产芽孢的细菌（JM-1070T 菌株）被分离为一株假定内生菌，来自健康玉米茎的内部茎组织。基于 16S rRNA 基因序列的系统发育分析将菌株 JM-1070T 置于粘杆菌属的单系群中，与粘杆菌属的模式种（相似度为 98%）和 Mucilaginibacter calamicampi（相似度为 97.2%）最为接近。与该属其他种的模式菌株的 16S rRNA 基因序列相似度为 93.4-96.0%。菌株 JM-1070T 与粘杆菌属模式菌株 M. composti LMG 23497T 和 M. calamicampi CCUG 63418T 之间的 DNA-DNA 杂交显示出较低的相关性，为 13%（相互 18%）和 52%（相互 54.4%）。主要呼吸醌为menaquinone MK-6 和 MK-7。主要脂肪酸（>15%）为 iso-C15:0、iso-C15:0 2-OH/C16:1ω7c（以总和特征 3 测量）和 iso-C17:0 3-OH。还检测到几种其他的支链和羟基脂肪酸。极性脂谱由主要成分磷脂酰乙醇胺和一种未鉴定的氨基磷脂组成。聚胺模式主要包含 sym-homospermidine。16S rRNA 基因测序、生理参数和聚胺、泛醌、极性脂和脂肪酸组成的特征表明，菌株 JM-1070T 代表粘杆菌属的一个新种。因此，我们建议使用 Mucilaginibacter auburnensis sp. nov. 来命名该菌株，其模式菌株为 JM-1070T（=CIP 110694T=LMG 28078T）。
