Sedláček Ivo, Pantůček Roman, Králová Stanislava, Mašlaňová Ivana, Holochová Pavla, Staňková Eva, Sobotka Roman, Barták Miloš, Busse Hans-Jürgen, Švec Pavel
Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Section of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4002-4007. doi: 10.1099/ijsem.0.002240. Epub 2017 Sep 14.
A bacterial strain designated CCM 8645 was isolated from a soil sample collected nearby a mummified seal carcass in the northern part of James Ross Island, Antarctica. The cells were short rods, Gram-stain-negative, non-motile, catalase and oxidase positive, and produced a red-pink pigment on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, extensive biotyping using conventional tests and commercial identification kits and chemotaxonomic analyses were applied to clarify its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene placed strain CCM 8645 in the genus Mucilaginibacter with the closest relative being Mucilaginibacter daejeonensis Jip 10, exhibiting 96.5 % 16S rRNA pairwise similarity which was clearly below the 97 % threshold value recommended for species demarcation. The major components in fatty acid profiles were Summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C15 : 0 iso and C17 : 0 iso 3OH. The cellular quinone content was exclusively menaquinone MK-7. The major polyamine was sym-homospermidine and predominant polar lipids were phosphatidylethanolamine and phosphatidylserine. Based on presented results, we propose a novel species for which the name Mucilaginibacter terrae sp. nov. is suggested, with the type strain CCM 8645 (=LMG 29437).
从南极洲詹姆斯罗斯岛北部一具海豹木乃伊尸体附近采集的土壤样本中分离出一株名为CCM 8645的细菌菌株。该菌细胞呈短杆状，革兰氏染色阴性，无运动性，过氧化氢酶和氧化酶呈阳性，在R2A琼脂上产生红粉色色素。采用基于16S rRNA基因测序的多相分类方法、使用传统试验和商业鉴定试剂盒进行广泛的生物分型以及化学分类分析来阐明其分类地位。基于16S rRNA基因的系统发育分析将菌株CCM 8645置于黏液杆菌属，与其亲缘关系最近的是大田黏液杆菌Jip 10，16S rRNA序列两两相似性为96.5%，明显低于物种划分推荐的97%阈值。脂肪酸谱中的主要成分是特征性总和3（C16 : 1ω7c/C16 : 1ω6c）、C15 : 0异戊酸和C17 : 0异戊酸3OH。细胞醌含量仅为甲基萘醌MK-7。主要多胺是对称高亚精胺，主要极性脂质是磷脂酰乙醇胺和磷脂酰丝氨酸。基于所呈现的结果，我们提议将其命名为陆地黏液杆菌新种（Mucilaginibacter terrae sp. nov.），模式菌株为CCM 8645（=LMG 29437）。
