Kämpfer Peter, Busse Hans-Jürgen, McInroy John A, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2015 Jul;65(7):2187-2193. doi: 10.1099/ijs.0.000236. Epub 2015 Apr 9.
A slightly yellow bacterial strain (JM-87(T)), isolated from the stem of healthy 10 day-old sweet corn (Zea mays), was studied for its taxonomic allocation. The isolate revealed Gram-stain-negative, rod-shaped cells. A comparison of the 16S rRNA gene sequence of the isolate showed 99.1, 97.8, and 97.4% similarity to the 16S rRNA gene sequences of the type strains of Elizabethkingia anophelis, Elizabethkingia meningoseptica and Elizabethkingia miricola, respectively. The fatty acid profile of strain JM-87(T) consisted mainly of the major fatty acids C15:0 iso, C17:0 iso 3-OH, and C15:0 iso 2-OH/C16:1ω7c/t. The quinone system of strain JM-87(T) contained, exclusively, menaquinone MK-6. The major polyamine was sym-homospermidine. The polar lipid profile consisted of the major lipid phosphatidylethanolamine plus several unidentified aminolipids and other unidentified lipids. DNA-DNA hybridization experiments with E. meningoseptica CCUG 214(T) ( = ATCC 13253(T)), E. miricola KCTC 12492(T) ( = GTC 862(T)) and E. anophelis R26(T) resulted in relatedness values of 17% (reciprocal 16%), 30% (reciprocal 19%), and 51% (reciprocal 54%), respectively. These DNA-DNA hybridization results, in addition to some differentiating biochemical properties, clearly indicate that strain JM-87(T) is a representative of a novel species, for which the name Elizabethkingia endophytica sp. nov. is proposed. The type strain is JM-87(T) ( = CIP 110885(T) = LMG 28604(T) = CCM 8570(T)).
从10日龄健康甜玉米（玉米）茎中分离出一株略带黄色的细菌菌株（JM-87(T)），对其进行分类归属研究。该分离株显示革兰氏染色阴性、杆状细胞。该分离株的16S rRNA基因序列与嗜蚊伊丽莎白菌、脑膜败血伊丽莎白菌和米氏伊丽莎白菌模式菌株的16S rRNA基因序列的相似性分别为99.1%、97.8%和97.4%。菌株JM-87(T)的脂肪酸谱主要由主要脂肪酸C15:0异戊酸、C17:0异戊酸3-OH和C15:0异戊酸2-OH/C16:1ω7c/t组成。菌株JM-87(T)的醌系统仅含有甲基萘醌MK-6。主要多胺是对称高亚精胺。极性脂质谱由主要脂质磷脂酰乙醇胺加上几种未鉴定的氨基脂质和其他未鉴定的脂质组成。与脑膜败血伊丽莎白菌CCUG 214(T)（=ATCC 13,253(T)）、米氏伊丽莎白菌KCTC 12,492(T)（=GTC 862(T)）和嗜蚊伊丽莎白菌R26(T)进行的DNA-DNA杂交实验，其相关性值分别为17%（相互16%）、30%（相互19%）和51%（相互54%）。这些DNA-DNA杂交结果，除了一些有区别的生化特性外，清楚地表明菌株JM-87(T)是一个新物种的代表，为此提出新名称内生伊丽莎白菌（Elizabethkingia endophytica sp. nov.）。模式菌株是JM-87(T)（=CIP 110,885(T)=LMG 28,604(T)=CCM 8,570(T)）。
