Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1259-1264. doi: 10.1099/ijs.0.032961-0. Epub 2011 Jul 15.
A bacterial strain, isolated from a sample of reef-building coral (Isopora palifera) collected off the coast of southern Taiwan, was characterized using a polyphasic taxonomic approach. The strain, designated sw-2(T), was Gram-staining-negative, aerobic, rod-shaped and motile, with subpolar flagella, and formed greyish pink colonies. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain sw-2(T) was most closely related to Roseivivax halodurans Och 239(T) (97.4 % sequence similarity) and Roseivivax halotolerans Och 210(T) (96.4 %). The novel strain did not require NaCl for growth and exhibited optimal growth at 35-40 °C, at pH 7.5-8.0 and with 3-7 % (w/v) NaCl. It produced bacteriochlorophyll a under aerobic conditions. Summed feature 8 (C(18:1)ω7c and/or C(18:1)ω6c; 63.7 %) predominated in the cellular fatty acid profile. The novel strain's major respiratory quinone was ubiquinone Q-10 and its genomic DNA G+C content was 68.8 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sulfo-quinovosyl diacylglycerol and three uncharacterized phospholipids. The level of DNA-DNA relatedness between strain sw-2(T) and Roseivivax halodurans Och 239(T) was only 15.0 %. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of the novel strain from all established species of the genus Roseivivax. Based on the genotypic, phenotypic and chemotaxonomic data, strain sw-2(T) represents a novel species in the genus Roseivivax, for which the name Roseivivax isoporae sp. nov. is proposed. The type strain is sw-2(T) ( = LMG 25204(T) = BCRC 17966(T)).
从台湾南部海岸采集的造礁珊瑚（Isopora palifera）样本中分离出的一株细菌，通过多相分类学方法进行了特征描述。该菌株被命名为 sw-2(T)，革兰氏染色阴性，需氧，杆状，运动，具有极性鞭毛，形成灰粉色菌落。基于 16S rRNA 基因序列的系统发育分析表明，菌株 sw-2(T)与 Roseivivax halodurans Och 239(T)（97.4%序列相似性）和 Roseivivax halotolerans Och 210(T)（96.4%序列相似性）最为密切相关。该新菌株的生长不需要 NaCl，最适生长温度为 35-40°C，pH 值为 7.5-8.0，NaCl 浓度为 3-7%（w/v）。它在有氧条件下产生细菌叶绿素 a。细胞脂肪酸图谱中以 summed feature 8（C(18:1)ω7c 和/或 C(18:1)ω6c；63.7%）为主。该新菌株的主要呼吸醌为泛醌 Q-10，基因组 DNA G+C 含量为 68.8mol%。极性脂谱由磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、双磷脂酰甘油、磷脂酰二甲乙醇胺、磺基-醌诺糖二酰甘油和三种未鉴定的磷脂组成。菌株 sw-2(T)与 Roseivivax halodurans Och 239(T)之间的 DNA-DNA 同源性水平仅为 15.0%。生理生化测试的结果允许该新菌株与 Roseivivax 属的所有已建立种进行清晰的表型区分。基于基因型、表型和化学分类学数据，菌株 sw-2(T)代表 Roseivivax 属的一个新种，建议将其命名为 Roseivivax isoporae sp. nov.。模式菌株为 sw-2(T)（=LMG 25204(T)=BCRC 17966(T)）。
