Polonelli L, Dettori G, Morace G, Rosa R, Castagnola M, Schipper M A
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia A. Gemelli, Università Cattolica del Sacro Cuore, Roma, Italy.
Antonie Van Leeuwenhoek. 1988;54(1):5-17. doi: 10.1007/BF00393954.
Fourteen isolates belonging to Rhizopus microsporus, Rh. rhizopodiformis, the progeny of Rh. microsporus x Rh. rhizopodiformis and an intermediate species, Rh. chinensis, were serologically tested either by a modification of the exoantigen technique of Kaufman & Standard (1978) or by the more analytical cross immunoelectrophoresis technique with intermediate gel. Common antigenic determinants in the isolates studied indicated that the species, their progeny and the intermediate species Rh. chinensis, are antigenically very closely related. Through absorptions, a specific reference antiserum for Rh. microsporus was obtained. With this adsorbed monospecific antiserum, using the exoantigen test, the identity of the isolates in study, determined after their general morphology as Rh. microsporus, was confirmed. For comparative purposes, the use of reversed phase high performance liquid chromatography for the investigation of fungal isolates is also reported.
对属于小孢根霉、根状根霉、小孢根霉与根状根霉的子代以及中间物种华根霉的14个分离株进行了血清学检测，检测方法为对考夫曼和斯坦德（1978年）的外抗原技术进行改良，或者采用更具分析性的中间凝胶交叉免疫电泳技术。所研究分离株中的共同抗原决定簇表明，这些物种、它们的子代以及中间物种华根霉在抗原性上密切相关。通过吸收试验，获得了小孢根霉的特异性参考抗血清。使用这种吸附的单特异性抗血清，通过外抗原试验，证实了所研究的分离株在根据其总体形态确定为小孢根霉后的身份。为了进行比较，还报道了使用反相高效液相色谱法对真菌分离株进行研究的情况。
