Nakakuma H, Arai M, Kawaguchi T, Horikawa K, Hidaka M, Sakamoto K, Iwamori M, Nagai Y, Takatsuki K
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
FEBS Lett. 1989 Dec 4;258(2):230-2. doi: 10.1016/0014-5793(89)81660-6.
A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin-layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the GalCers, the mAb reacted with teh two GalCers which contained alpha-hydroxy fatty acids, but not with GalCer composed of nonhydroxy fatty acids. These findings suggest that not only that the mAb discriminated the fatty acid composition in the ceramide moiety of GalCer, but also that the ceramide structure defines the immunological epitope as it is known to do for the carbohydrate moiety of glycosphingolipid.
开发了一种针对单己糖神经酰胺的小鼠单克隆抗体（mAb）。该单克隆抗体在薄层色谱图上与从牛脑中获得的3种半乳糖基神经酰胺（GalCer）有不同反应。对这3种糖脂的结构分析表明，它们由相同的半乳糖和鞘氨醇组成，但脂肪酸明显不同。在GalCer中，该单克隆抗体与含有α-羟基脂肪酸的两种GalCer反应，但不与由非羟基脂肪酸组成的GalCer反应。这些发现表明，该单克隆抗体不仅能区分GalCer神经酰胺部分的脂肪酸组成，而且神经酰胺结构如同已知对糖鞘脂碳水化合物部分所起的作用那样，定义了免疫表位。
