Fukuda Eriko, Uesawa Yoshihiro, Baba Masaki, Okada Yoshihito
Department of Natural Medicine and Phytochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Department of Clinical Pharmaceutics, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Nat Prod Commun. 2013 Dec;8(12):1721-4.
In order to identify Glycyrrhiza species by chemical fingerprinting, the bark of the roots and stolons of Glycyrrhiza uralensis Fischer and G. glabra Linné were analyzed using DART (Direct Analysis in Real Time)-MS. The characteristic peaks of each species were determined statistically by volcano plot. This summarizes the relationship between the p-values of a statistical test and the magnitude of the difference in values of the samples in the groups. In this experiment, peaks that had a p value <0.05 in the t test and Z2 absolute difference were defined as characteristic. As a result, characteristic peaks of G. uralensis were found at m/z 299, 315, 341, and 369. In contrast, characteristic peaks of G. glabra were found at m/z 323, 325, 337, 339, and 391. In conclusion, we found several characteristic peaks to distinguish G. uralensis and G. glabra by DART-MS using volcano plot. This method can be applied to identify the Glycyrrhiza species.
为了通过化学指纹图谱鉴定甘草属植物，使用实时直接分析质谱法（DART-MS）对乌拉尔甘草（Glycyrrhiza uralensis Fischer）和光果甘草（G. glabra Linné）的根皮及匍匐茎进行了分析。通过火山图对每个物种的特征峰进行了统计学确定。这总结了统计检验的p值与组内样本值差异大小之间的关系。在本实验中，t检验中p值<0.05且Z2绝对差异的峰被定义为特征峰。结果，在乌拉尔甘草中发现了m/z 299、315、341和369处的特征峰。相比之下，在光果甘草中发现了m/z 323、325、337、339和391处的特征峰。总之，我们通过使用火山图的DART-MS发现了几个区分乌拉尔甘草和光果甘草的特征峰。该方法可用于鉴定甘草属植物。
