Kang Sang-Ho, Lee Jeong-Hoon, Lee Hyun Oh, Ahn Byoung Ohg, Won So Youn, Sohn Seong-Han, Kim Jung Sun
Genomics Division, National Institute of Agricultural Sciences.
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science.
Genes Genet Syst. 2018 Oct 30;93(3):83-89. doi: 10.1266/ggs.17-00002. Epub 2017 Oct 6.
Glycyrrhiza uralensis and G. glabra, members of the Fabaceae, are medicinally important species that are native to Asia and Europe. Extracts from these plants are widely used as natural sweeteners because of their much greater sweetness than sucrose. In this study, the three complete chloroplast genomes and five 45S nuclear ribosomal (nr)DNA sequences of these two licorice species and an interspecific hybrid are presented. The chloroplast genomes of G. glabra, G. uralensis and G. glabra × G. uralensis were 127,895 bp, 127,716 bp and 127,939 bp, respectively. The three chloroplast genomes harbored 110 annotated genes, including 76 protein-coding genes, 30 tRNA genes and 4 rRNA genes. The 45S nrDNA sequences were either 5,947 or 5,948 bp in length. Glycyrrhiza glabra and G. glabra × G. uralensis showed two types of nrDNA, while G. uralensis contained a single type. The complete 45S nrDNA sequence unit contains 18S rRNA, ITS1, 5.8S rRNA, ITS2 and 26S rRNA. We identified simple sequence repeat and tandem repeat sequences. We also developed four reliable markers for analysis of Glycyrrhiza diversity authentication.
豆科的乌拉尔甘草和光果甘草是原产于亚洲和欧洲的重要药用植物。由于这些植物的提取物比蔗糖甜得多，因此被广泛用作天然甜味剂。在本研究中，我们展示了这两种甘草及其种间杂种的三个完整叶绿体基因组和五个45S核糖体（nr）DNA序列。光果甘草、乌拉尔甘草和光果甘草×乌拉尔甘草的叶绿体基因组分别为127,895 bp、127,716 bp和127,939 bp。这三个叶绿体基因组共包含110个注释基因，其中包括76个蛋白质编码基因、30个tRNA基因和4个rRNA基因。45S nrDNA序列长度为5,947或5,948 bp。光果甘草和光果甘草×乌拉尔甘草表现出两种nrDNA类型，而乌拉尔甘草只包含一种类型。完整的45S nrDNA序列单元包含18S rRNA、ITS1、5.8S rRNA、ITS2和26S rRNA。我们鉴定了简单序列重复和串联重复序列。我们还开发了四个可靠的标记用于甘草多样性鉴定分析。
