Pitt T L
Division of Hospital Infection, Central Public Health Laboratory, London, UK.
Eur J Clin Microbiol Infect Dis. 1988 Apr;7(2):238-47. doi: 10.1007/BF01963095.
Current knowledge of the typing of Pseudomonas aeruginosa and new methods for characterizing strains are reviewed. A combination of serotyping as a primary screen with pyocin typing for finer discrimination between isolates gives valid epidemiological data, is within the scope of most clinical laboratories and is to be recommended. Phage typing and H typing do provide good discrimination but are not reproducible in practice because a reaction-difference rule has to be applied to phage patterns, and diphasic variation reduces the accuracy of the identification of flagellar antigens. A problem remains with cystic fibrosis isolates, which show considerable heterogeneity in surface properties. For these strains pyocin production typing by the revised method of Govan is indicated. Newer techniques such as isoenzyme profile and DNA probes of the chromosome require independent evaluation and due to their technical difficulty may not be adopted in a routine context for some years to come.
本文综述了铜绿假单胞菌分型的现有知识以及菌株特征鉴定的新方法。将血清分型作为初步筛选，结合绿脓菌素分型以更精细地区分分离株，可提供有效的流行病学数据，大多数临床实验室都能做到，值得推荐。噬菌体分型和H分型确实能提供良好的区分，但在实际操作中不可重复，因为必须对噬菌体模式应用反应差异规则，且双相变异会降低鞭毛抗原鉴定的准确性。囊性纤维化分离株仍存在问题，其表面特性表现出相当大的异质性。对于这些菌株，建议采用戈万修订方法进行绿脓菌素产生分型。诸如同工酶谱和染色体DNA探针等较新的技术需要独立评估，由于其技术难度，在未来几年内可能无法在常规情况下采用。
