使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析铜绿假单胞菌10/b型绿脓菌素临床分离株的周质蛋白以进行亚型分类。

The use of SDS polyacrylamide gel electrophoresis of periplasmic proteins to subtype Pseudomonas aeruginosa pyocin type 10/b clinical isolates.

作者信息

Adwan K

机构信息

Dept. of Biological Sciences, An-Najah National University, Nablus, Palestine.

出版信息

Infection. 1999 Jan-Feb;27(1):39-41. doi: 10.1007/BF02565170.

DOI:10.1007/BF02565170

PMID:10027106

Abstract

The periplasmic protein banding patterns (PPBPs) of thirteen strains of Pseudomonas aeruginosa pyocin type 10/b implicated in two nosocomial outbreaks in the neonatal unit of Rafeidia Hospital, Nablus, Palestine, were examined. In addition, five strains from sporadic cases from the same unit occurring in 1996 and 1997 were also studied. Despite different sources of the strains, PPBPs generated by PAGE suggested a clonal nature of the strains obtained during each of the two outbreaks. Although they had very similar PPBPs, the two outbreak clones were not identical. In contrast, sporadic strains of P. aeruginosa pyocin type 10 appeared to be much more heterogeneous than those of the two outbreaks. PPBP analysis appeared to be a useful tool that may be of value for epidemiological purposes.

摘要

对涉及巴勒斯坦纳布卢斯拉菲迪亚医院新生儿病房两起医院感染暴发的13株10/b型绿脓杆菌绿脓菌素的周质蛋白条带模式（PPBP）进行了检测。此外，还研究了1996年和1997年该病房散发病例中的5株菌株。尽管菌株来源不同，但聚丙烯酰胺凝胶电泳（PAGE）产生的PPBP表明，两次暴发期间获得的菌株具有克隆性质。虽然两个暴发克隆的PPBP非常相似，但并不完全相同。相比之下，10型绿脓杆菌绿脓菌素的散发病菌株似乎比两次暴发中的菌株更加多样化。PPBP分析似乎是一种有用的工具，可能对流行病学研究有价值。