Haak-Frendscho M, Arai N, Arai K, Baeza M L, Finn A, Kaplan A P
Division of Allergy, Rheumatology, and Clinical Immunology, State University of New York, Stony Brook 11798-8161.
J Clin Invest. 1988 Jul;82(1):17-20. doi: 10.1172/JCI113567.
Histamine-releasing factors (HRFs) have been shown to be released from a variety of human cells, including T lymphocytes and alveolar macrophages. We considered the possibility that known cytokines might possess such activity on human basophils and/or mast cells and therefore tested preparations of human recombinant IL 3, IL 4, IL 5, granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) upon a panel of basophil donors. IL 3 and GM-CSF possessed significant histamine-releasing activity in 8 of 10 and 12 of 14 subjects, respectively. In each instance, a dose response could be demonstrated. IL 4 and G-CSF had no such activity, whereas IL 5 had activity in only 2 of 14 donors tested. We conclude that IL 3 and GM-CSF represent two effective HRFs, and suggest that HRF, as isolated based upon histamine-releasing activity, is likely to be heterogeneous in terms of molecular identity. Whether previously described HRFs relate specifically to IL 3 or GM-CSF must await primary sequence analysis of HRF and/or studies with monospecific antisera.
组胺释放因子（HRFs）已被证明可从多种人类细胞中释放出来，包括T淋巴细胞和肺泡巨噬细胞。我们考虑了已知细胞因子可能对人类嗜碱性粒细胞和/或肥大细胞具有这种活性的可能性，因此在一组嗜碱性粒细胞供体上测试了重组人白细胞介素3（IL 3）、白细胞介素4（IL 4）、白细胞介素5（IL 5）、粒细胞集落刺激因子（G-CSF）和粒细胞巨噬细胞集落刺激因子（GM-CSF）的制剂。IL 3和GM-CSF分别在10名受试者中的8名和14名受试者中的12名中具有显著的组胺释放活性。在每种情况下，都可以证明剂量反应。IL 4和G-CSF没有这种活性，而IL 5仅在14名受试供体中的2名中具有活性。我们得出结论，IL 3和GM-CSF代表两种有效的HRFs，并表明基于组胺释放活性分离的HRF在分子身份方面可能是异质的。先前描述的HRFs是否与IL 3或GM-CSF特异性相关，必须等待HRF的一级序列分析和/或用单特异性抗血清进行的研究。
