Dewalick Saskia, Hensbergen Paul J, Bexkens Michiel L, Grosserichter-Wagener Christina, Hokke Cornelis H, Deelder André M, de Groot Philip G, Tielens Aloysius G M, van Hellemond Jaap J
Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Int J Parasitol. 2014 Apr;44(5):263-8. doi: 10.1016/j.ijpara.2013.12.006. Epub 2014 Feb 18.
Schistosoma mansoni eggs have to cross the endothelium and intestinal wall to leave the host and continue the life cycle. Mechanisms involved in this essential step are largely unknown. Here we describe direct binding to the S. mansoni eggshell of von Willebrand factor and other plasma proteins involved in haemostasis. Using deletion-mutants, we demonstrated that it is the A1 domain of von Willebrand factor that binds to the eggshell. Our results suggest that binding of plasma proteins to the eggshell promotes binding to the endothelium, initiating the passage of the egg through the blood-vessel wall to be excreted in the end.
曼氏血吸虫卵必须穿过内皮和肠壁才能离开宿主并继续其生命周期。这一关键步骤所涉及的机制在很大程度上尚不清楚。在这里，我们描述了血管性血友病因子和其他参与止血的血浆蛋白与曼氏血吸虫卵壳的直接结合。使用缺失突变体，我们证明了与卵壳结合的是血管性血友病因子的A1结构域。我们的结果表明，血浆蛋白与卵壳的结合促进了与内皮的结合，从而启动卵穿过血管壁并最终排出体外的过程。
